Name : Human IL-6 Protein
Product Source :
Recombinant Human IL-6 is expressed from E.coli without tag. It contains Val30-Met212.[Accession | P05231]
Molecular Weight :
The protein has a predicted 20.8 kDa same as Tris-Bis PAGE result.
Endotoxin Level :
Less than 1EU per μg by the LAL method.
Purity :
> 95% as determined by Tris-Bis PAGE> 90% as determined by HPLC
Formulation :
Lyophilized from 0.22μm filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.
Reconstitution :
Centrifuge the tube before opening. Reconstituting to a concentration more than 100 μg/ml is recommended. Dissolve the lyophilized protein in distilled water.
Storage and Stability :
-20 to -80°C for 12 months as supplied from date of receipt. -80°C for 3-6 months after reconstitution. 2-8°C for 2-7 days after reconstitution. Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.
Product Concentration :
Tris-Bis PAGE Human IL-6 on Tris-Bis PAGE under reduced condition. The purity is greater than 95%. SEC-HPLC The purity of Human IL-6 is greater than 90% as determined by SEC-HPLC. ELISA Data Immobilized Human IL-6 at 0.5μg/ml (100μl/Well) on the plate. Dose response curve for Human IL-6 R alpha, hFc Tag with the EC50 48.7ng/ml determined by ELISA. SPR Data Human IL-6 R alpha, His Tag captured on CM5 Chip via anti-his antibody can bind Human IL-6, No Tag with an affinity constant of 0.22 nM as determined in SPR assay (Biacore T200). Cell Based Assay Measured in a cell proliferation assay using TF‑1 human erythroleukemic cells. The ED50 for this effect is 0.1 – 0.5 ng/mL (QC Test).
Background :
IL-6 (Interleukin-6) is a pleiotropic cytokine that acts in the acute phase reaction, inflammation, hematopoiesis, bone metabolism, and cancer progression.Interleukin 6 has been shown to interact with interleukin-6 receptor and glycoprotein.
Synonyms :
IFN-beta-2; IL6; IL-6; BSF-2; CDF; MGI-2A; Interleukin-6; HSF; IFNB2; HGF;IFN-β-2
References & Citations :
(1)Gabay C. Interleukin-6 and chronic inflammation[J]. Arthritis Research & Therapy, 2006, 8.
Related category websites: https://www.medchemexpress.com/recombinant-proteins.html
Popular product recommendations:
TGF beta 1/TGFB1 Protein
BCAS2 Protein
Popular categories:
Chemokine Receptor
Interleukin & Receptors