Name : Human IL-7R alpha/CD127 Protein
Product Source :
Recombinant Human IL-7R alpha/CD127 Protein is expressed from HEK293 with hFc tag at the C-Terminus. It contains Glu21-Gly236.[Accession | P16871-1]
Molecular Weight :
The protein has a predicted MW of 51.6 kDa. Due to glycosylation, the protein migrates to 65-75 kDa based on Tris-Bis PAGE result.
Endotoxin Level :
Less than 1EU per μg by the LAL method.
Purity :
> 95% as determined by Tris-Bis PAGE> 95% as determined by HPLC
Formulation :
Lyophilized from 0.22μm filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.
Reconstitution :
Centrifuge the tube before opening. Reconstituting to a concentration more than 100 μg/ml is recommended. Dissolve the lyophilized protein in distilled water.
Storage and Stability :
-20 to -80°C for 12 months as supplied from date of receipt. -80°C for 3-6 months after reconstitution. 2-8°C for 2-7 days after reconstitution. Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.
Product Concentration :
Tris-Bis PAGE Human IL-7R alpha on Tris-Bis PAGE under reduced condition. The purity is greater than 95%. SEC-HPLC The purity of Human IL-7R alpha is greater than 95% as determined by SEC-HPLC. ELISA Data Immobilized Human IL-7 at 0.5μg/ml (100μl/Well) on the plate. Dose response curve for Human IL-7R alpha, hFc Tag with the EC50 of 52.5ng/ml determined by ELISA.
Background :
Interleukin 7 (IL-7) and its receptor (IL-7R, a heterodimer of IL-7Rα and γc) are essential for normal lymphoid development. IL-7 and IL-7R activate three main pathways: STAT5, PI3K/Akt/mTOR and MEK/Erk, ultimately leading to the promotion of leukemia cell viability, cell cycle progression and growth.
Synonyms :
IL-17R alpha; IL7Ra; CD127; CDW127; ILRA
References & Citations :
(1)Oliveira ML, Akkapeddi P, Ribeiro D, Melão A, Barata JT. IL-7R-mediated signaling in T-cell acute lymphoblastic leukemia: An update. Adv Biol Regul. 2019 Jan;71:88-96. doi: 10.1016/j.jbior.2018.09.012. Epub 2018 Sep 19. PMID: 30249539; PMCID: PMC6386770.
Related category websites: https://www.medchemexpress.com/recombinant-proteins.html
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