Name : Mouse IL-17A&F Protein
Product Source :
Recombinant Mouse IL-17A&F Protein is expressed from HEK293 with His tag and Avi tag at the C-Terminus. It contains Ala26-Ala158(IL-17A)&Arg29-Ala161(IL-17F).[Accession | Q62386(IL-17A)&Q7TNI7-1(IL-17F)]
Molecular Weight :
The protein has a predicted MW of 17.9 kDa(IL-17A)&14.9 kDa(IL-17F). Due to glycosylation, the protein migrates to 22-25 kDa&27-30 kDa based on Tris-Bis PAGE result.
Endotoxin Level :
Less than 1EU per μg by the LAL method.
Purity :
> 95% as determined by Tris-Bis PAGE> 95% as determined by HPLC
Formulation :
Lyophilized from 0.22μm filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.
Reconstitution :
Centrifuge the tube before opening. Reconstituting to a concentration more than 100 μg/ml is recommended. Dissolve the lyophilized protein in distilled water.
Storage and Stability :
-20 to -80°C for 12 months as supplied from date of receipt.-80°C for 3-6 months after reconstitution.2-8°C for 2-7 days after reconstitution.Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.
Product Concentration :
Tris-Bis PAGE Mouse IL-17A&F on Tris-Bis PAGE under reduced condition. The purity is greater than 95%. SEC-HPLC The purity of Mouse IL-17A&F is greater than 95% as determined by SEC-HPLC. ELISA Data Immobilized Mouse IL-17A&F, His Tag at 1μg/ml (100μl/well) on the plate. Dose response curve for Mouse IL-17R alpha, hFc Tag with the EC50 of 35.9ng/ml determined by ELISA.
Background :
Interleukin‑17A (IL‑17A), also known as CTLA‑8, is a 15‑20 kDa glycosylated cytokine that plays an important role in anti‑microbial and chronic inflammation. The six IL‑17 cytokines (IL‑17A‑F) are encoded by separate genes but adopt a conserved cystine knot fold.IL-17A is a ligand for IL17RA and IL17RC. The heterodimer formed by IL17A and IL17F is a ligand for the heterodimeric complex formed by IL17RA and IL17RC. Involved in inducing stromal cells to produce proinflammatory and hematopoietic cytokines.
Synonyms :
IL-17A;Interleukin-17A;CTLA-8;IL-17;IL-17F;IL24;ML-1;Interleukin-17F
References & Citations :
(1)Gaffen S L. An overview of IL-17 function and signaling[J]. Cytokine, 2008, 43(3):0-407.
Related category websites: https://www.medchemexpress.com/recombinant-proteins.html
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