Name : Cynomolgus IL-18RAP Protein
Product Source :
Recombinant Cynomolgus IL-18RAP Protein is expressed from HEK293 with His tag at the C-Terminus. It contains Phe20-Arg356.[Accession | A0A2K5VDC3]
Molecular Weight :
The protein has a predicted MW of 39.5 kDa. Due to glycosylation, the protein migrates to 60-68 kDa based on Tris-Bis PAGE result.
Endotoxin Level :
Less than 1EU per μg by the LAL method.
Purity :
> 95% as determined by Tris-Bis PAGE> 95% as determined by HPLC
Formulation :
Lyophilized from 0.22μm filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.
Reconstitution :
Centrifuge the tube before opening. Reconstituting to a concentration more than 100 μg/ml is recommended. Dissolve the lyophilized protein in distilled water.
Storage and Stability :
-20 to -80°C for 12 months as supplied from date of receipt.-80°C for 3-6 months after reconstitution.2-8°C for 2-7 days after reconstitution.Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.
Product Concentration :
Tris-Bis PAGE Cynomolgus IL-18RAP on Tris-Bis PAGE under reduced condition. The purity is greater than 95%. SEC-HPLC The purity of Cynomolgus IL-18RAP is greater than 95% as determined by SEC-HPLC. ELISA Data Immobilized Cynomolgus IL-18RAP, His Tag at 0.2μg/ml (100μl/well) on the plate. Dose response curve for Anti-IL-18RAP Antibody, hFc Tag with the EC50 of 3.5ng/ml determined by ELISA.
Background :
IL-18RAP, also known as IL-18Rβ or IL-1R7, is component of IL-18 receptor complex and its interactions with IL-18R1 mediate signal transduction initiated by IL-18 although it does not directly bind IL-18 in vitro.
Synonyms :
ACPL; CD218b; CDw218b; IL-18R-beta; IL-18Rbeta; IL-18RAcP; IL-1R-7; IL-1R7; IL-1RAcPL; IL18RB; IL18RAP; IL-18R-beta; IL-18R-β; IL-18Rβ
References & Citations :
(1)Hedl M, Zheng S, Abraham C. The IL18RAP region disease polymorphism decreases IL-18RAP/IL-18R1/IL-1R1 expression and signaling through innate receptor-initiated pathways. J Immunol. 2014;192(12):5924-5932. doi:10.4049/jimmunol.1302727(2)Zhu J, Liu C, Teng X, Yin J, Zheng L, Wang L, Tang W, Gu H, Gu B, Chen L. Association of the interleukin-18 receptor 1 and interleukin-18 receptor accessory protein polymorphisms with the risk of esophageal cancer. Biomed Rep. 2016 Feb;4(2):227-235.
Related category websites: https://www.medchemexpress.com/recombinant-proteins.html
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