Name : Cynomolgus IL-4 R alpha/CD124 Protein
Product Source :
Recombinant Cynomolgus IL-4 R alpha/CD124 Protein is expressed from HEK293 with His tag at the C-Terminus. It contains Met26-Arg232.[Accession | G7Q0S7]
Molecular Weight :
The protein has a predicted MW of 24.84 kDa. Due to glycosylation, the protein migrates to 45-60 kDa based on Tris-Bis PAGE result.
Endotoxin Level :
Less than 1EU per μg by the LAL method.
Purity :
> 95% as determined by Tris-Bis PAGE> 95% as determined by HPLC
Formulation :
Lyophilized from 0.22μm filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.
Reconstitution :
Centrifuge the tube before opening. Reconstituting to a concentration more than 100 μg/ml is recommended. Dissolve the lyophilized protein in distilled water.
Storage and Stability :
-20 to -80°C for 12 months as supplied from date of receipt.-80°C for 3-6 months after reconstitution.2-8°C for 2-7 days after reconstitution.Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.
Product Concentration :
Cynomolgus IL-4 R alpha on Tris-Bis PAGE under reduced condition. The purity is greater than 95%. The purity of Cynomolgus IL-4 R alpha is greater than 95% as determined by SEC-HPLC. ELISA Data Immobilized Human IL-4, No Tag at 2μg/ml (100μl/well) on the plate. Dose response curve for Cynomolgus IL-4 R alpha, His Tag with the EC50 of 91.8ng/ml determined by ELISA (QC Test). SPR Data Cynomolgus IL-4, His Tag immobilized on CM5 Chip can bind Cynomolgus IL-4 R alpha, His Tag with an affinity constant of 13.48 nM as determined in SPR assay (Biacore T200).
Background :
Interleukin 4 Receptor alpha (IL-4 Ra), also known as CD124 and BSF receptor, is a widely expressed 140 kDa transmembrane glycoprotein in the class I cytokine receptor family.The effects of IL-4 are mediated after binding to high affinity receptor complexes present on hematopoietic as well as non-hematopoietic cells.
Synonyms :
CD124; IL-4 R alpha; IL-4R subunit alpha; IL4R; IL-4Ra; IL4RACD124
References & Citations :
(1)Ghoreschi K , Thomas P , Breit S , et al. Interleukin-4 therapy of psoriasis induces Th2 responses and improves human autoimmune disease[J]. Nature Medicine, 2002, 9(1):40-46.
Related category websites: https://www.medchemexpress.com/recombinant-proteins.html
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