Name : Human IL-9 Protein
Product Source :
Recombinant Human IL-9 Protein is expressed from HEK293 with His tag at the C-Terminus. It contains Gln19-Ile144.[Accession | P15248]
Molecular Weight :
The protein has a predicted MW of 15.3 kDa. Due to glycosylation, the protein migrates to 40-50 kDa based on Tris-Bis PAGE result.
Endotoxin Level :
Less than 1EU per μg by the LAL method.
Purity :
> 95% as determined by Tris-Bis PAGE> 95% as determined by HPLC
Formulation :
Lyophilized from 0.22μm filtered solution in 20mM Tris, 150mM NaCl (pH 7.5). Normally 8% trehalose is added as protectant before lyophilization.
Reconstitution :
Centrifuge the tube before opening. Reconstituting to a concentration more than 100 μg/ml is recommended. Dissolve the lyophilized protein in distilled water.
Storage and Stability :
-20 to -80°C for 12 months as supplied from date of receipt.-80°C for 3-6 months after reconstitution.2-8°C for 2-7 days after reconstitution.Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.
Product Concentration :
Tris-Bis PAGE Human IL-9 on Tris-Bis PAGE under reduced condition. The purity is greater than 95%. SEC-HPLC The purity of Human IL-9 is greater than 95% as determined by SEC-HPLC. ELISA Data Immobilized Human IL-9, His Tag at 0.2μg/ml (100μl/Well) on the plate. Dose response curve for Anti-IL-9 Anitibody, hFc Tag with the EC50 of 13.6ng/ml determined by ELISA. Cell Based Assay Measured in a cell proliferation assay using MO7e human megakaryocytic leukemic cells. The ED50 for this effect is
Background :
IL-9 is a pleiotropic cytokine that influences various distinct functions of different target cells such as T cells, B cells, mast cells and airway epithelial cells by activating STAT1, STAT3 and STAT5. Because of its pleiotropic functions, IL-9 has been demonstrated to be involved in several diseases, such as cancer, autoimmunity and other pathogen-mediated immune-regulated diseases. In this review, we focus on the role of Th9 and IL-9-producing cells in allergic asthma.
Synonyms :
Interleukin-9; IL-9; Cytokine P40; T-cell growth factor P40; HP40; P40
References & Citations :
(1) Koch S, Sopel N, Finotto S. Th9 and other IL-9-producing cells in allergic asthma. Semin Immunopathol. 2017 Jan;39(1):55-68. doi: 10.1007/s00281-016-0601-1. Epub 2016 Nov 17. PMID: 27858144.
Related category websites: https://www.medchemexpress.com/recombinant-proteins.html
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