Name : Human Adenylosuccinate Lyase Protein
Product Source :
Recombinant Human Adenylosuccinate Lyase Protein is expressed from E.coli with His tag at the N-Terminus. It contains Ala2-Leu484.[Accession | P30566-1]
Molecular Weight :
The protein has a predicted MW of 56.28 kDa same as Tris-Bis PAGE result.
Endotoxin Level :
Less than 1EU per μg by the LAL method.
Purity :
> 95% as determined by Tris-Bis PAGE
Formulation :
Lyophilized from 0.22μm filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.
Reconstitution :
Centrifuge the tube before opening. Reconstituting to a concentration more than 100 μg/ml is recommended. Dissolve the lyophilized protein in distilled water.
Storage and Stability :
-20 to -80°C for 12 months as supplied from date of receipt.-80°C for 3-6 months after reconstitution.2-8°C for 2-7 days after reconstitution.Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.
Product Concentration :
Tris-Bis PAGE Human Adenylosuccinate Lyase on Tris-Bis PAGE under reduced condition. The purity is greater than 95%.
Background :
Adenylosuccinate lyase (ADSL) is an essential enzyme for de novo purine biosynthesis, ADSL functions in de novo purine synthesis (DNPS) and the purine nucleotide cycle. Adenylosuccinate lyase ADSL) deficiency is a defect of purine metabolism affecting purinosome assembly and reducing metabolite fluxes through purine de novo synthesis and purine nucleotide recycling pathways.
Synonyms :
ADSL; ASase; Adenylosuccinase; AMPS; ASL
References & Citations :
(1) Taha-Mehlitz S, Bianco G, Coto-Llerena M, Kancherla V, Bantug GR, Gallon J, Ercan C, Panebianco F, Eppenberger-Castori S, von Strauss M, Staubli S, Bolli M, Peterli R, Matter MS, Terracciano LM, von Flüe M, Ng CKY, Soysal SD, Kollmar O, Piscuoglio S. Adenylosuccinate lyase is oncogenic in colorectal cancer by causing mitochondrial dysfunction and independent activation of NRF2 and mTOR-MYC-axis. Theranostics. 2021 Feb 15;11(9):4011-4029. doi: 10.7150/thno.50051. PMID: 33754045; PMCID: PMC7977451.
Related category websites: https://www.medchemexpress.com/recombinant-proteins.html
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