Name : Human IL-17A/CTLA-8 Protein

Product Source :
Recombinant Human IL-17A/CTLA-8 Protein is expressed from HEK293 with His tag at the C-Terminus. It contains Gly24-Ala155.[Accession | Q16552-1]

Molecular Weight :
The protein has a predicted MW of 18.2 kDa. Due to glycosylation, the protein migrates to 16-30 kDa based on Tris-Bis PAGE result.

Endotoxin Level :
Less than 1EU per μg by the LAL method.

Purity :
> 95% as determined by Tris-Bis PAGE> 95% as determined by HPLC

Formulation :
Lyophilized from 0.22μm filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.

Reconstitution :
Centrifuge the tube before opening. Reconstituting to a concentration more than 100 μg/ml is recommended. Dissolve the lyophilized protein in distilled water.

Storage and Stability :
-20 to -80°C for 12 months as supplied from date of receipt. -80°C for 3-6 months after reconstitution. 2-8°C for 2-7 days after reconstitution. Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.

Product Concentration :
Tris-Bis PAGE Human IL-17A on Tris-Bis PAGE under reduced condition. The purity is greater than 95%. SEC-HPLC The purity of Human IL-17A is greater than 95% as determined by SEC-HPLC. ELISA Data Immobilized Human IL-17A, His Tag at 0.5μg/ml (100μl/well) on the plate. Dose response curve for Anti-IL-17A Antibody, hFc Tag with the EC50 of 4.6ng/ml determined by ELISA (QC Test). ELISA Data Immobilized Human IL-17A, His Tag at 1μg/ml (100μl/well) on the plate. Dose response curve for Human IL-17R alpha, hFc Tag with the EC50 of 12.4ng/ml determined by ELISA (Routine Test). ELISA Data Immobilized Human IL-17A, His Tag at 1μg/ml (100μl/Well) on the plate. Dose response curve for Human IL-17Rc, hFc Tag with the EC50 of 60.6ng/ml determined by ELISA (Routine Test). SPR Data Human IL-17R alpha, His Tag immobilized on CM5 Chip can bind Human IL-17A, His Tag with an affinity constant of 1.57nM as determined in SPR assay (Biacore T200) (Routine Test).

Background :
Interleukin‑17A (IL‑17A), also known as CTLA‑8, is a 15‑20 kDa glycosylated cytokine that plays an important role in anti‑microbial and chronic inflammation. The six IL‑17 cytokines (IL‑17A‑F) are encoded by separate genes but adopt a conserved cystine knot fold.IL-17A is a ligand for IL17RA and IL17RC. The heterodimer formed by IL17A and IL17F is a ligand for the heterodimeric complex formed by IL17RA and IL17RC. Involved in inducing stromal cells to produce proinflammatory and hematopoietic cytokines.

Synonyms :
CTLA-8; IL17; IL17A; IL-17CTLA-8; interleukin 17A

References & Citations :
(1)Gaffen S L. An overview of IL-17 function and signaling[J]. Cytokine, 2008, 43(3):0-407.

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