Name : Human IL-27RA/TCCR Protein
Product Source :
Recombinant Human IL-27RA/TCCR Protein is expressed from HEK293 with hFc tag at the C-Terminus. It contains Gly34-Lys516.[Accession | Q6UWB1]
Molecular Weight :
The protein has a predicted MW of 79.3 kDa. Due to glycosylation, the protein migrates to 90-120 kDa based on Tris-Bis PAGE result.
Endotoxin Level :
Less than 1EU per μg by the LAL method.
Purity :
> 95% as determined by Tris-Bis PAGE
Formulation :
Lyophilized from 0.22μm filtered solution in PBS, 5mM DTT (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.
Reconstitution :
Centrifuge the tube before opening. Reconstituting to a concentration more than 100 μg/ml is recommended. Dissolve the lyophilized protein in distilled water.
Storage and Stability :
-20 to -80°C for 12 months as supplied from date of receipt.-80°C for 3-6 months after reconstitution.2-8°C for 2-7 days after reconstitution.Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.
Product Concentration :
Tris-Bis PAGE Human IL-27RA on Tris-Bis PAGE under reduced condition. The purity is greater than 95%. ELISA Data Immobilized Human IL-27 RA, hFc Tag at 5μg/ml (100μl/well) on the plate. Dose response curve for Human IL-27, His Tag with the EC50 of 55.9ng/ml determined by ELISA (QC Test). SPR Data Human IL-27 RA, hFc Tag captured on CM5 Chip via Protein A can bind Human IL-27, His Tag with an affinity constant of 3.58 nM as determined in SPR assay (Biacore T200).
Background :
A multicenter, web-based Thyroid Cancer and Tumor Collaborative Registry (TCCR, http://tccr.unmc.edu) allows for the collection and management of various data on thyroid cancer (TC) and thyroid nodule (TN) patients. The TCCR is coupled with OpenSpecimen, an open-source biobank management system, to annotate biospecimens obtained from the TCCR subjects.
Synonyms :
IL-27RA; TCCR; IL27RA; IL-27R-alpha; IL27R alpha; WSX-1; WSX1; ZcytoR1; CRL1; IL-27 RA; IL27 RA
References & Citations :
(1)Shats O, Goldner W, Feng J, Sherman A, Smith RB, Sherman S. Thyroid Cancer and Tumor Collaborative Registry (TCCR). Cancer Inform. 2016 May 3;15:73-9. doi: 10.4137/CIN.S32470. PMID: 27168721; PMCID: PMC4856228.
Related category websites: https://www.medchemexpress.com/recombinant-proteins.html
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