Name : Biotinylated Human IL-5 R alpha/CD125 Protein
Product Source :
Recombinant Biotinylated Human IL-5 R alpha/CD125 Protein is expressed from HEK293 with His tag and Avi tag at the C-Terminus. It contains Asp21-Glu335.[Accession | Q01344-1]
Molecular Weight :
The protein has a predicted MW of 38.7 kDa. Due to glycosylation, the protein migrates to 55-60 kDa based on Tris-Bis PAGE result.
Endotoxin Level :
Less than 1EU per ug by the LAL method.
Purity :
> 95% as determined by Tris-Bis PAGE> 95% as determined by HPLC
Formulation :
Lyophilized from 0.22μm filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.
Reconstitution :
Centrifuge the tube before opening. Reconstituting to a concentration more than 100 μg/ml is recommended. Dissolve the lyophilized protein in distilled water.
Storage and Stability :
-20 to -80°C for 12 months as supplied from date of receipt. -80°C for 3-6 months after reconstitution. 2-8°C for 2-7 days after reconstitution. Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.
Product Concentration :
Tris-Bis PAGE Biotinylated Human IL-5 R alpha on Tris-Bis PAGE under reduced condition. The purity is greater than 95%. SEC-HPLC The purity of Biotinylated Human IL-5 R alpha is greater than 95% as determined by SEC-HPLC. ELISA Data Immobilized Human IL-5, His Tag at 0.5μg/ml (100μl/well) on the plate. Dose response curve for Biotinylated Human IL-5 R alpha, His Tag with the EC50 of 0.12μg/ml determined by ELISA.
Background :
Interleukin‑5 Receptor alpha (IL‑5 R alpha), also known as CD125, is a 60 kDa hematopoietin receptor that plays a dominant role in eosinophil biology.This is the receptor for interleukin-5. The alpha chain binds to IL5.
Synonyms :
CD125; CDw125CD125; HSIL5R3; IL-5 R alpha; IL-5R subunit alpha; IL5R; IL5RA; IL-5Ra; MGC26560
References & Citations :
(1)Garcia G , Taille C , Laveneziana P , et al. Anti-interleukin-5 therapy in severe asthma[J]. European Respiratory Review, 2013, 22(129):251-257.
Related category websites: https://www.medchemexpress.com/recombinant-proteins.html
Popular product recommendations:
PLTP Protein
Intrinsic Factor/GIF Protein
Popular categories:
CCL14
Immunoglobulin Superfamily Member 11 (IGSF11)