Name : Biotinylated Human IL-6 R alpha/CD126 Protein
Product Source :
Recombinant Biotinylated Human IL-6 R alpha/CD126 Protein is expressed from HEK293 with His tag and Avi tag at the C-Terminus. It contains Leu20-Pro365.[Accession | P08887-1]
Molecular Weight :
The protein has a predicted MW of 41.4 kDa. Due to glycosylation, the protein migrates to 70-80 kDa based on Tris-Bis PAGE result.
Endotoxin Level :
Less than 1EU per μg by the LAL method.
Purity :
> 95% as determined by Tris-Bis PAGE> 95% as determined by HPLC
Formulation :
Lyophilized from 0.22μm filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.
Reconstitution :
Centrifuge the tube before opening. Reconstituting to a concentration more than 100 μg/ml is recommended. Dissolve the lyophilized protein in distilled water.
Storage and Stability :
-20 to -80°C for 12 months as supplied from date of receipt. -80°C for 3-6 months after reconstitution. 2-8°C for 2-7 days after reconstitution. Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.
Product Concentration :
Tris-Bis PAGE Biotinylated Human IL-6 R alpha on Tris-Bis PAGE under reduced condition. The purity is greater than 95%. SEC-HPLC The purity of Biotinylated Human IL-6 R alpha is greater than 95% as determined by SEC-HPLC. ELISA Data Immobilized Human IL-6, No Tag at 2μg/ml (100μl/well) on the plate. Dose response curve for Biotinylated Human IL-6 R alpha, His Tag with the EC50 of 1.04μg/ml determined by ELISA.
Background :
The multifunctional factor interleukin 6 (IL-6) exerts its activities through binding to a high-affinity receptor complex consisting of two membrane glycoproteins: an 80 kDa component receptor that binds IL-6 with low affinity (IL-6R alpha) and a signal-transducing component of 130 kDa (gp130) that does not bind IL-6 by itself, but is required for high-affinity binding of IL-6 by the complex. Both components of the receptor complex, IL-6R alpha and gp130 have been cloned, sequenced, and expressed.
Synonyms :
IL-6R subunit alpha; IL-6R-alpha; IL-6RA; IL-6R 1; IL6R; CD126; IL6RQ; gp80
References & Citations :
(1)Schmidt-Arras D , Rose-John S. IL-6 pathway in the liver: from physiopathology to therapy[J]. Journal of Hepatology, 2016:S0168827816000830.D
Related category websites: https://www.medchemexpress.com/recombinant-proteins.html
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