Name : Human Integrin alpha 2B beta 3 (ITGA2B&ITGB3) Heterodimer Protein
Product Source :
Recombinant Human Integrin alpha 2B beta 3 (ITGA2B&ITGB3) Heterodimer Protein is expressed from HEK293 with His tag at the C-Terminus. It contains Leu32-Arg993(ITGA2B) acidic tail & GLy27-Asp718(ITGB3) basic tail.[Accession | P08514-1(ITGA2B)&P05106-1(ITGB3)]
Molecular Weight :
The protein has a predicted MW of 110.5 kDa (ITGA2B)&81.3 kDa (ITGB3). Due to glycosylation, the protein migrates to 95-115 kDa based on Tris-Bis PAGE result.
Endotoxin Level :
Less than 1EU per μg by the LAL method.
Purity :
> 95% as determined by Tris-Bis PAGE> 95% as determined by HPLC
Formulation :
Lyophilized from 0.22μm filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.
Reconstitution :
Centrifuge the tube before opening. Reconstituting to a concentration more than 100 μg/ml is recommended. Dissolve the lyophilized protein in distilled water.
Storage and Stability :
-20 to -80°C for 12 months as supplied from date of receipt.-80°C for 3-6 months after reconstitution.2-8°C for 2-7 days after reconstitution.Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.
Product Concentration :
Tris-Bis PAGE Human ITGA2B&ITGB3 on Tris-Bis PAGE under reduced condition. The purity is greater than 95%. SEC-HPLC The purity of Human ITGA2B&ITGB3 is greater than 95% as determined by SEC-HPLC.
Background :
Glanzmann thrombasthenia (GT) is characterized by mucocutaneous bleeding due to platelets that fail to aggregate in response to physiologic stimuli. GT, a rare inherited disease, is caused by quantitative or qualitative deficiencies of αIIbβ3, an integrin receptor for adhesive proteins. Coded by the ITGA2B and ITGB3 genes, αIIbβ3 mediates platelet-to-platelet attachment, aggregation and clot retraction.
Synonyms :
α2Bβ3; ITGA2B&ITGB3; ITGA2B; ITGB3
References & Citations :
(1)Nurden AT, Pillois X, Nurden P. Understanding the genetic basis of Glanzmann thrombasthenia: implications for treatment. Expert Rev Hematol. 2012 Oct;5(5):487-503. doi: 10.1586/ehm.12.46. PMID: 23146053.
Related category websites: https://www.medchemexpress.com/recombinant-proteins.html
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