Name : Human Integrin alpha M beta 2 (ITGAM&ITGB2) Heterodimer Protein
Product Source :
Recombinant Human Integrin alpha M beta 2 (ITGAM&ITGB2) Heterodimer Protein is expressed from HEK293 with His tag at the C-Terminus. It contains Phe17-Asn1104(ITGAM) acidic tail&Gln23-Asn700(ITGB2) basic tail.[Accession | P11215-1(ITGAM)&P05107-1(ITGB2)]
Molecular Weight :
The protein has a predicted MW of 126.10 kDa(ITGAM)&79.50 kDa(ITGB2). Due to glycosylation, the protein migrates to 140-160 kDa (ITGAM)&90-100 kDa (ITGB2) based on Tris-Bis PAGE result.
Endotoxin Level :
Less than 1EU per μg by the LAL method.
Purity :
> 95% as determined by Tris-Bis PAGE> 95% as determined by HPLC
Formulation :
Lyophilized from 0.22μm filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.
Reconstitution :
Centrifuge the tube before opening. Reconstituting to a concentration more than 100 μg/ml is recommended. Dissolve the lyophilized protein in distilled water.
Storage and Stability :
-20 to -80°C for 12 months as supplied from date of receipt.-80°C for 3-6 months after reconstitution.2-8°C for 2-7 days after reconstitution.Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.
Product Concentration :
Tris-Bis PAGE Human ITGAM&ITGB2 on Tris-Bis PAGE under reduced condition. The purity is greater than 95%. SEC-HPLC The purity of Human ITGAM&ITGB2 is greater than 95% as determined by SEC-HPLC. ELISA Data Immobilized Human ITGAM&ITGB2, His Tag at 1μg/ml (100μl/well) on the plate. Dose response curve for Anti-ITGB2 Antibody, hFc Tag with the EC50 of 13.7ng/ml determined by ELISA.
Background :
Integrin αMβ2, also called Mac-1 or complement receptor-3 (CR3), is a heterodimer expressed on monocytes. It functions as both an adhesion molecule mediating the diapedesis of leukocytes across the endothelium and a receptor for the iC3b fragment of complement responsible for phagocytic/degranulation responses to microorganisms[1]. Active αMβ2 binds iC3b, coagulation proteins fibrinogen, plasminogen and factor X, extracellular matrix (ECM) proteins fibronectin, laminin and collagen, and cell surface ICAMs, myelin basic protein and DCSIGN[2].
Synonyms :
αMβ2; ITGAM&ITGB2; ITGAM; ITGB2
References & Citations :
[1] Ross GD. Regulation of the adhesion versus cytotoxic functions of the Mac-1/CR3/alphaMbeta2-integrin glycoprotein. Crit Rev Immunol. 2000;20(3):197-222.
Related category websites: https://www.medchemexpress.com/recombinant-proteins.html
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