Name : Biotinylated Human Integrin alpha 5 beta 1 (ITGA5&ITGB1) Heterodimer Protein
Product Source :
Recombinant Biotinylated Human Integrin alpha 5 beta 1 (ITGA5&ITGB1) Heterodimer Protein is expressed from HEK293 with His tag and Avi tag at the C-Terminus. It contains Phe42-Tyr995(ITGA5) acidic tail&Gln21-Asp728(ITGB1) basic tail.[Accession | P08648(ITGA5)&P05556-1(ITGB1)]
Molecular Weight :
The protein has a predicted MW of 111.8 kDa (ITGA5)&83.2 kDa (ITGB1). Due to glycosylation, the protein migrates to 110-140 kDa based on Tris-Bis PAGE result.
Endotoxin Level :
Less than 1EU per μg by the LAL method.
Purity :
> 95% as determined by Tris-Bis PAGE> 90% as determined by HPLC
Formulation :
Lyophilized from 0.22μm filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.
Reconstitution :
Centrifuge the tube before opening. Reconstituting to a concentration more than 100 μg/ml is recommended. Dissolve the lyophilized protein in distilled water.
Storage and Stability :
-20 to -80°C for 12 months as supplied from date of receipt.-80°C for 3-6 months after reconstitution.2-8°C for 2-7 days after reconstitution.Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.
Product Concentration :
Tris-Bis PAGE Biotinylated Human ITGA5&ITGB1 on Tris-Bis PAGE under reduced condition. The purity is greater than 95%. SEC-HPLC The purity of Biotinylated Human ITGA5&ITGB1 is greater than 90% as determined by SEC-HPLC. ELISA Data Immobilized 0.5μg Anti-ITGA5&ITGB1 Antibody, hFc Tag at 0.5μg/ml (100μl/Well) on the plate. Dose response curve for Biotinylated Human ITGA5&ITGB1, His Tag with the EC50 of 55.3ng/ml determined by ELISA.
Background :
Integrin alpha 5/ beta 1, also known as VLA-5, is a widely expressed non-covalent heterodimer of a 160 kDa alpha 5 and a 130 kDa beta 1 Integrin subunit.Alpha 5/ beta 1 is up‑regulated on tumor vasculature and promotes angiogenesis.
Synonyms :
Integrin alpha 5 beta 1; VLA-5; ITGA5&ITGB1; Integrin alpha 5 β 1; α5β1
References & Citations :
(1)Zhang Z , Vuori K , Reed J C , et al. The alpha5beta1 Integrin Supports Survival of Cells on Fibronectin and Up-Regulates Bcl2 Expression[J]. Proceedings of the National Academy of Sciences, 1995, 92(13):6161-6165.
Related category websites: https://www.medchemexpress.com/recombinant-proteins.html
Popular product recommendations:
Siglec-15 Protein
IL-17F Protein
Popular categories:
Death Receptor 5
Estrogen Receptor