Name : Human ITCH Protein
Product Source :
Recombinant Human ITCH Protein is expressed from E.coli without tag. It contains Arg485-Glu862.[Accession | NP_113671.3]
Molecular Weight :
The protein has a predicted MW of 44.98 kDa same as Tris-Bis PAGE result.
Endotoxin Level :
Less than 1EU per μg by the LAL method.
Purity :
> 95% as determined by Tris-Bis PAGE> 90% as determined by HPLC
Formulation :
Lyophilized from 0.22 μm filtered solution in PBS, 200mM L-Arginine (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.
Reconstitution :
Centrifuge the tube before opening. Reconstituting to a concentration more than 100 μg/ml is recommended. Dissolve the lyophilized protein in distilled water.
Storage and Stability :
-20 to -80°C for 12 months as supplied from date of receipt. -80°C for 3-6 months after reconstitution. 2-8°C for 2-7 days after reconstitution. Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.
Product Concentration :
Tris-Bis PAGE Human ITCH on Tris-Bis PAGE under reduced condition. The purity is greater than 95%. SEC-HPLC The purity of Human ITCH is greater than 90% as determined by SEC-HPLC.
Background :
Itch or itchy E3 is a member of the homologous to E6-associated protein C-terminus (HECT)-type family of E3 ligases, with the protein-interacting WW-domains for the recruitment of substrate and the HECT domain for the transfer of ubiquitin to the substrate, and its deletion results in an itchy phenotype with constant skin scratching and multi-organ inflammation.
Synonyms :
AIP4; NAPP1; AIF4
References & Citations :
Aki D, Zhang W, Liu YC. The E3 ligase Itch in immune regulation and beyond. Immunol Rev. 2015 Jul;266(1):6-26. doi: 10.1111/imr.12301. PMID: 26085204.
Related category websites: https://www.medchemexpress.com/recombinant-proteins.html
Popular product recommendations:
PSME1 Protein
Animal-Free IFN-lambda 2/IL-28A Protein
Popular categories:
Receptor Tyrosine Phosphatase
MUC-24/CD164