Name : Human ITK Protein
Product Source :
Recombinant Human ITK Protein is expressed from Baculovirus-Insect Cells with GST tag at the N-terminus. It contains Arg352-Leu620.[Accession | NP_005537.3]
Molecular Weight :
The protein has a predicted MW of 57.33 kDa same as Tris-Bis PAGE result.
Endotoxin Level :
Less than 1EU per μg by the LAL method.
Purity :
> 95% as determined by Tris-Bis PAGE
Formulation :
Supplied as 0.22 μm filtered solution in 50mM Tris, 150mM NaCl, 25% glycerol, 0.5mM PMSF, 0.5 mM TCEP, 0.1mM EDTA, 0.5mM GSH (pH 7.5).
Reconstitution :
Storage and Stability :
Valid for 12 months from date of receipt when stored at -80°C. Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.
Product Concentration :
Tris-Bis PAGE Human ITK on Tris-Bis PAGE under reduced condition. The purity is greater than 95%.
Background :
Itk/Emt, a tec family tyrosine kinase, is important for T-cell development and activation through the antigen receptor. Itk/Emt can induce the generation of important second signals (PKC, Ca2+) and can modulate their duration by maintaining the stability of scaffolding complexes through regulation of cytoskeletal reorganization.
Synonyms :
Kinase EMT; ITK; EMT; LYK; T-cell-specific kinase;
References & Citations :
Tsoukas CD, Grasis JA, Ching KA, Kawakami Y, Kawakami T. Itk/Emt: a link between T cell antigen receptor-mediated Ca2+ events and cytoskeletal reorganization. Trends Immunol. 2001 Jan;22(1):17-20. doi: 10.1016/s1471-4906(00)01795-6. PMID: 11286686.
Related category websites: https://www.medchemexpress.com/recombinant-proteins.html
Popular product recommendations:
IL-6 Protein
ZG16B Protein
Popular categories:
IFN-lambda 2/IL-28A
PPAR gamma