Name : Mouse ITK Protein

Product Source :
Recombinant Mouse ITK Protein is expressed from Baculovirus-Insect Cells with His tag and GST tag at the N-terminus and Strep-II tag at the C-terminus. It contains Arg347-Leu625.[Accession | Q03526]

Molecular Weight :
The protein has a predicted MW of 61.42 kDa same as Tris-Bis PAGE result.

Endotoxin Level :
Less than 1EU per μg by the LAL method.

Purity :
> 95% as determined by Tris-Bis PAGE

Formulation :
Supplied as 0.22 μm filtered solution in 50mM Tris, 100mM NaCl, 10% Glycerol, 0.5mM GSH (pH 7.5).

Reconstitution :

Storage and Stability :
Valid for 12 months from date of receipt when stored at -80°C. Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.

Product Concentration :
Tris-Bis PAGE Mouse ITK on Tris-Bis PAGE under reduced condition. The purity is greater than 95%.

Background :
ITK (IL-2-inducible tyrosine kinase) belongs to the Tec family kinases and is mainly expressed in T cells. It is involved in TCR signalling events driving processes like T cell development as well as Th2, Th9 and Th17 responses thereby controlling the expression of pro-inflammatory cytokines.

Synonyms :
Kinase EMT; Kinase TLK; Emt; Tlk; Tsk; Tcsk

References & Citations :
Lechner KS, Neurath MF, Weigmann B. Role of the IL-2 inducible tyrosine kinase ITK and its inhibitors in disease pathogenesis. J Mol Med (Berl). 2020 Oct;98(10):1385-1395. doi: 10.1007/s00109-020-01958-z. Epub 2020 Aug 18. PMID: 32808093; PMCID: PMC7524833.

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