Name : Mouse JAM-A Protein
Product Source :
Recombinant Mouse JAM-A Protein is expressed from HEK293 with His tag at the C-Terminus. It contains Lys27-Gly238.[Accession | O88792]
Molecular Weight :
The protein has a predicted MW of 23.9 kDa. Due to glycosylation, the protein migrates to 28-35 kDa based on Tris-Bis PAGE result.
Endotoxin Level :
Less than 1EU per μg by the LAL method.
Purity :
> 95% as determined by Tris-Bis PAGE
Formulation :
Lyophilized from 0.22μm filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.
Reconstitution :
Centrifuge the tube before opening. Reconstituting to a concentration more than 100 μg/ml is recommended. Dissolve the lyophilized protein in distilled water.
Storage and Stability :
-20 to -80°C for 12 months as supplied from date of receipt. -80°C for 3-6 months after reconstitution. 2-8°C for 2-7 days after reconstitution. Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.
Product Concentration :
Tris-Bis PAGE Mouse JAM-A on Tris-Bis PAGE under reduced condition. The purity is greater than 95%.
Background :
junctional adhesion molecule A (JAM-A), a cell adhesion molecule, is highly elevated in human GBM cancer stem cells and predicts poor patient prognosis. While JAM-A is also highly expressed in other cells in the tumor microenvironment, specifically microglia and macrophages,JAM-A functions to suppress pathogenic microglial activation in the female tumor microenvironment, highlighting an emerging role for sex differences in the GBM microenvironment and suggesting that sex differences extend beyond previously reported tumor cell-intrinsic differences.
Synonyms :
JAM-A; JAM-1; CD321; JAM; JCAM; JCAM1; KAT; PAM-1; F11 receptor; F11R; PAM-1KAT
References & Citations :
(1) Turaga SM, Silver DJ, Bayik D, Paouri E, Peng S, Lauko A, Alban TJ, Borjini N, Stanko S, Naik UP, Keri RA, Connor JR, Barnholtz-Sloan JS, Rubin JB, Berens M, Davalos D, Lathia JD. JAM-A functions as a female microglial tumor suppressor in glioblastoma. Neuro Oncol. 2020 Nov 26;22(11):1591-1601. doi: 10.1093/neuonc/noaa148. PMID: 32592484; PMCID: PMC7690368.
Related category websites: https://www.medchemexpress.com/recombinant-proteins.html
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