Name : Mouse Beta Klotho Protein
Product Source :
Recombinant Mouse Beta Klotho Protein is expressed from HEK293 with His tag at the C-Terminus. It contains Phe53-Pro994.[Accession | Q99N32-1]
Molecular Weight :
The protein has a predicted MW of 109.91 kDa. Due to glycosylation, the protein migrates to 120-130 kDa based on Tris-Bis PAGE result.
Endotoxin Level :
Less than 1EU per μg by the LAL method.
Purity :
> 90% as determined by Tris-Bis PAGE> 90% as determined by HPLC
Formulation :
Supplied as 0.22μm filtered solution in PBS (pH 7.4).
Reconstitution :
Storage and Stability :
Valid for 12 months from date of receipt when stored at -80°C. Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.
Product Concentration :
Tris-Bis PAGE Mouse Beta Klotho on Tris-Bis PAGE under reduced condition. The purity is greater than 90%. SEC-HPLC The purity of Mouse Beta Klotho Protein is greater than 90% as determined by SEC-HPLC. SPR Data Human FGFR4, hFc Tag captured on CM5 Chip via Protein A can bind Mouse Beta Klotho, His Tag with an affinity constant of 36.97 nM as determined in SPR assay (Biacore T200).
Background :
Beta-klotho (KLB) is a coreceptor required for endocrine fibroblast growth factor (FGF) 15/19 and FGF21 signaling in the brain. Klb is prominent within the hypothalamus, which is consistent with its metabolic functions, but diverse roles for Klb are now emerging. Central Klb expression is low but discrete and may govern FGF-targeted sites.
Synonyms :
betaKlotho; beta-klotho; BKL; KLB; klotho beta like; Klotho beta; MGC142213;βKlotho; β-klotho; klotho β like; Klotho β
References & Citations :
(1)Bono BS, Koziel Ly NK, Miller PA, Williams-Ikhenoba J, Dumiaty Y, Chee MJ. Spatial distribution of beta-klotho mRNA in the mouse hypothalamus, hippocampal region, subiculum, and amygdala. J Comp Neurol. 2022 Feb 10. doi: 10.1002/cne.25306. Epub ahead of print. PMID: 35143049.
Related category websites: https://www.medchemexpress.com/recombinant-proteins.html
Popular product recommendations:
LRG1 Protein
RANTES/CCL5 Protein
Popular categories:
HIV-1 gp120
OX40