Name : Human Kallikrein 5/KLK5 Protein
Product Source :
Recombinant Human Kallikrein 5/KLK5 Protein is expressed from HEK293 with His tag at the C-Terminus. It contains Val23-Ser293.[Accession | Q9Y337]
Molecular Weight :
The protein has a predicted MW of 30.7 kDa. Due to glycosylation, the protein migrates to 42-48 kDa based on Tris-Bis PAGE result.
Endotoxin Level :
Less than 1EU per μg by the LAL method.
Purity :
> 95% as determined by Tris-Bis PAGE> 95% as determined by HPLC
Formulation :
Lyophilized from 0.22μm filtered solution in 50mM MES, 150mM NaCl (pH 5.5). Normally 8% trehalose is added as protectant before lyophilization.
Reconstitution :
Centrifuge the tube before opening. Reconstituting to a concentration more than 100 μg/ml is recommended. Dissolve the lyophilized protein in 50mM MES, 150mM NaCl (pH 5.5).
Storage and Stability :
-20 to -80°C for 12 months as supplied from date of receipt.-80°C for 3-6 months after reconstitution.2-8°C for 2-7 days after reconstitution.Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.
Product Concentration :
Tris-Bis PAGE Human Kallikrein 5 on Tris-Bis PAGE under reduced condition. The purity is greater than 95%. SEC-HPLC The purity of Human Kallikrein 5 is greater than 95% as determined by SEC-HPLC. ELISA Data Immobilized Human Kallikrein 5, His Tag at 0.5μg/ml (100μl/Well) on the plate. Dose response curve for Anti-Kallikrein 5 Antibody, hFc Tag with the EC50 of 9.4ng/ml determined by ELISA.
Background :
The inhibition of kallikrein 5 (KLK5) has been identified as a potential strategy for treatment of the genetic skin disorder Netherton syndrome, in which loss-of-function mutations in the SPINK5 gene lead to down-regulation of the endogenous inhibitor LEKTI-1 and profound skin-barrier defects with severe allergic manifestations. To aid in the development of a medicine for this target, an X-ray crystallographic system was developed to facilitate fragment-guided chemistry and knowledge-based drug-discovery approaches.
Synonyms :
Kallikrein c; Klnc; KLK5; KLKL2; KLK-L2; SCTE
References & Citations :
(1) Thorpe JH, Edgar EV, Smith KJ, Lewell XQ, Rella M, White GV, Polyakova O, Nassau P, Walker AL, Holmes DS, Pearce AC, Wang Y, Liddle J, Hovnanian A. Evaluation of a crystallographic surrogate for kallikrein 5 in the discovery of novel inhibitors for Netherton syndrome. Acta Crystallogr F Struct Biol Commun. 2019 May 1;75(Pt 5):385-391. doi: 10.1107/S2053230X19003169. Epub 2019 Apr 26. PMID: 31045568; PMCID: PMC6497096.
Related category websites: https://www.medchemexpress.com/recombinant-proteins.html
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