Name : Human KLKB1 Protein
Product Source :
Recombinant Human KLKB1 Protein is expressed from HEK293 with His tag at the C-Terminus. It contains Gly20-Ala638.[Accession | P03952]
Molecular Weight :
The protein has a predicted MW of 70.3 kDa. Due to glycosylation, the protein migrates to 72-80 kDa based on Tris-Bis PAGE result.
Endotoxin Level :
Less than 1EU per μg by the LAL method.
Purity :
> 95% as determined by Tris-Bis PAGE> 95% as determined by HPLC
Formulation :
Lyophilized from 0.22μm filtered solution in 20mM NaAc,150mM NaCl (pH 5.0). Normally 8% trehalose is added as protectant before lyophilization.
Reconstitution :
Centrifuge the tube before opening. Reconstituting to a concentration more than 100 μg/ml is recommended. Dissolve the lyophilized protein in 20mM NaAc,150mM NaCl (pH 5.0).
Storage and Stability :
-20 to -80°C for 12 months as supplied from date of receipt.-80°C for 3-6 months after reconstitution.2-8°C for 2-7 days after reconstitution.Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.
Product Concentration :
Tris-Bis PAGE Human KLKB1 on Tris-Bis PAGE under reduced condition. The purity is greater than 95%. SEC-HPLC The purity of Human KLKB1 is greater than 95% as determined by SEC-HPLC. Bioactivity Data Measured by its ability to cleave a flourogenic peptide substrate Pro-Phe-Arg-7-amido-4-methylcoumarin (PFR-AMC). The specific activity is > 1000 pmol/min/μg.
Background :
Plasma kallikrein, also known as Fletcher factor or kallikrein B1 (KLKB1), is a serine endopeptidase, like its homologs tissue kallikrein and kallikrein-related peptidases (KLKs). Its physiological role is to catalyze the release of kinins and other vasoactive peptides.
Synonyms :
Plasma kallikrein; PKK; KLK3; KLKB1; Fletcher factor; Kininogenin
References & Citations :
(1) Adamopoulos PG, Kontos CK, Papageorgiou SG, Pappa V, Scorilas A. KLKB1 mRNA overexpression: A novel molecular biomarker for the diagnosis of chronic lymphocytic leukemia. Clin Biochem. 2015 Sep;48(13-14):849-54. doi: 10.1016/j.clinbiochem.2015.04.007. Epub 2015 Apr 17. PMID: 25891023.
Related category websites: https://www.medchemexpress.com/recombinant-proteins.html
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