Name : Human Kremen-1 Protein
Product Source :
Recombinant Human Kremen-1 Protein is expressed from HEK293 with hFc tag at the C-Terminus. It contains Arg21-Thr392.[Accession | Q96MU8-1]
Molecular Weight :
The protein has a predicted MW of 67.6 kDa. Due to glycosylation, the protein migrates to 80-110 kDa based on Tris-Bis PAGE result.
Endotoxin Level :
Less than 1EU per μg by the LAL method.
Purity :
> 95% as determined by Tris-Bis PAGE> 95% as determined by HPLC
Formulation :
Lyophilized from 0.22μm filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.
Reconstitution :
Centrifuge the tube before opening. Reconstituting to a concentration more than 100 μg/ml is recommended. Dissolve the lyophilized protein in distilled water.
Storage and Stability :
-20 to -80°C for 12 months as supplied from date of receipt.-80°C for 3-6 months after reconstitution.2-8°C for 2-7 days after reconstitution.Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.
Product Concentration :
Tris-Bis PAGE Human Kremen-1 on Tris-Bis PAGE under reduced condition. The purity is greater than 95%. SEC-HPLC The purity of Human Kremen-1 is greater than 95% as determined by SEC-HPLC.
Background :
KREMEN1 (KRM1) has been identified as a functional receptor for Coxsackievirus A10 (CV-A10), a causative agent of hand-foot-and-mouth disease (HFMD), which poses a great threat to infants globally. KRM1 selectively binds to the mature viral particle above the canyon of the viral protein 1 (VP1) subunit and contacts across two adjacent asymmetry units. The key residues for receptor binding are conserved among most KRM1-dependent enteroviruses, suggesting a uniform mechanism for receptor binding.
Synonyms :
Kremen protein 1; KREMEN; KRM1; KREMEN1
References & Citations :
(1) Cui Y, Peng R, Song H, Tong Z, Qu X, Liu S, Zhao X, Chai Y, Wang P, Gao GF, Qi J. Molecular basis of Coxsackievirus A10 entry using the two-in-one attachment and uncoating receptor KRM1. Proc Natl Acad Sci U S A. 2020 Aug 4;117(31):18711-18718. doi: 10.1073/pnas.2005341117. Epub 2020 Jul 20. PMID: 32690697; PMCID: PMC7414063.
Related category websites: https://www.medchemexpress.com/recombinant-proteins.html
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