Name : Human Kremen-2 Protein
Product Source :
Recombinant Human Kremen-2 Protein is expressed from HEK293 with His tag at the C-Terminus. It contains Gly26-Ala364.[Accession | Q8NCW0-1]
Molecular Weight :
The protein has a predicted MW of 37.1 kDa. Due to glycosylation, the protein migrates to 50-60 kDa based on Tris-Bis PAGE result.
Endotoxin Level :
Less than 1EU per μg by the LAL method.
Purity :
> 95% as determined by Tris-Bis PAGE> 95% as determined by HPLC
Formulation :
Lyophilized from 0.22μm filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.
Reconstitution :
Centrifuge the tube before opening. Reconstituting to a concentration more than 100 μg/ml is recommended. Dissolve the lyophilized protein in distilled water.
Storage and Stability :
-20 to -80°C for 12 months as supplied from date of receipt.-80°C for 3-6 months after reconstitution.2-8°C for 2-7 days after reconstitution.Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.
Product Concentration :
Tris-Bis PAGE Human Kremen-2 on Tris-Bis PAGE under reduced condition. The purity is greater than 95%. SEC-HPLC The purity of Human Kremen-2 is greater than 95% as determined by SEC-HPLC. ELISA Data Immobilized Human Kremen-2, His Tag at 0.5μg/ml (100μl/well) on the plate. Dose response curve for Anti-Kremen-2 Antibody, hFc Tag with the EC50 of 5.4ng/ml determined by ELISA.
Background :
Kremen2 (Krm2) plays an important role in embryonic development, bone formation, and tumorigenesis as a crucial regulator of classical Wnt/β-catenin signaling pathway. Compared to para-cancerous tissues, Krm2 was significantly up-regulated in gastric cancer tissues and was positively correlated with the pathological grade of gastric cancer patients. Krm2 can be a potent candidate for designing of targeted therapy.
Synonyms :
KRM2; KREMEN2; Kremen-2; Dickkopf receptor 2
References & Citations :
(1) Chen B, Wang SQ, Huang J, Xu W, Lv H, Nie C, Wang J, Zhao H, Liu Y, Li J, Lu C, Zhang J, Chen XB. Knockdown of Kremen2 Inhibits Tumor Growth and Migration in Gastric Cancer. Front Oncol. 2021 Jan 7;10:534095. doi: 10.3389/fonc.2020.534095. PMID: 33489867; PMCID: PMC7817645.
Related category websites: https://www.medchemexpress.com/recombinant-proteins.html
Popular product recommendations:
NUP210 Protein
IL-17A Protein
Popular categories:
Cadherins
BDCA-4/CD304