Name : Human LAG3/CD223 Protein
Product Source :
Recombinant Human LAG3/CD223 Protein is expressed from HEK293 with His tag at the C-Terminus. It contains Leu23-Gly434.[Accession | P18627-1]
Molecular Weight :
The protein has a predicted MW of 45.5 kDa. Due to glycosylation, the protein migrates to 55-65 kDa based on Tris-Bis PAGE result.
Endotoxin Level :
Less than 1EU per μg by the LAL method.
Purity :
> 95% as determined by Tris-Bis PAGE> 95% as determined by HPLC
Formulation :
Lyophilized from 0.22μm filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.
Reconstitution :
Centrifuge the tube before opening. Reconstituting to a concentration more than 100 μg/ml is recommended. Dissolve the lyophilized protein in distilled water.
Storage and Stability :
-20 to -80°C for 12 months as supplied from date of receipt. -80°C for 3-6 months after reconstitution. 2-8°C for 2-7 days after reconstitution. Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.
Product Concentration :
Tris-Bis PAGE Human LAG3 on Tris-Bis PAGE under reduced condition. The purity is greater than 95%. SEC-HPLC The purity of Human LAG3 is greater than 95% as determined by SEC-HPLC. ELISA Data Immobilized Human LAG3, His Tag at 0.5μg/ml (100μl/well) on the plate. Dose response curve for Anti-LAG3 Antibody, hFc Tag with the EC50 of 9.1ng/ml determined by ELISA (QC Test). ELISA Data Immobilized Human LAG3, His Tag at 2μg/ml (100μl/Well) on the plate. Dose response curve for Human FGL1, hFc Tag with the EC50 of 0.91μg/ml determined by ELISA.
Background :
LAG-3, is a protein which in humans is encoded by the LAG3 gene, which is a cell surface molecule with diverse biologic effects on T cell function. It is an immune checkpoint receptor and as such is the target of various drug development programs by pharmaceutical companies seeking to develop new treatments for cancer and autoimmune disorders.
Synonyms :
CD223; FDC; LAG3; LAG-3; sLAG-3
References & Citations :
(1)Wang, J., Sanmamed, M.F., Datar, I., Su, T.T., Ji, L., Sun, J., Chen, L., Chen, Y., Zhu, G., Yin, W., et al. (2019). Fibrinogen-like Protein 1 Is a Major Immune Inhibitory Ligand of LAG-3. Cell 176, 334-347.e312.
Related category websites: https://www.medchemexpress.com/recombinant-proteins.html
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