Name : Human LAIR1/CD305 Protein
Product Source :
Recombinant Human LAIR1/CD305 Protein is expressed from HEK293 with His tag and Avi tag at the C-Terminus. It contains Gln22-His163.[Accession | Q6GTX8-1]
Molecular Weight :
The protein has a predicted MW of 18.2 kDa. Due to glycosylation, the protein migrates to 30-36 kDa based on Tris-Bis PAGE result.
Endotoxin Level :
Less than 1EU per μg by the LAL method.
Purity :
> 95% as determined by Tris-Bis PAGE> 95% as determined by HPLC
Formulation :
Lyophilized from 0.22μm filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.
Reconstitution :
Centrifuge the tube before opening. Reconstituting to a concentration more than 100 μg/ml is recommended. Dissolve the lyophilized protein in distilled water.
Storage and Stability :
-20 to -80°C for 12 months as supplied from date of receipt. -80°C for 3-6 months after reconstitution. 2-8°C for 2-7 days after reconstitution. Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.
Product Concentration :
Tris-Bis PAGE Human LAIR1 on Tris-Bis PAGE under reduced conditions. The purity is greater than 95%. SEC-HPLC The purity of Human LAIR1 is greater than 95% as determined by SEC-HPLC. ELISA Data Immobilized Human LAIR1, His Tag at 0.5μg/ml (100μl/well) on the plate. Dose response curve for Anti-LAIR1 Antibody, hFc Tag with the EC50 of 3.0ng/ml determined by ELISA (QC Test). SPR Data Human LAIR1, His Tag captured on CM5 Chip via Anti-His Antibody can bind Native Human Collagen I protein with an affinity constant of 0.082 nM as determined in SPR assay (Biacore T200).
Background :
LAIR1 (leukocyte-associated Ig-like receptor-1, designated CD305) is an approximately 40 kDa type I transmembrane inhibitory glycoprotein belonging to the Ig superfamily.LAIR1 functions as an inhibitory receptor that plays a constitutive negative regulatory role on cytolytic function of natural killer (NK) cells, B-cells and T-cells. Activation by Tyr phosphorylation results in recruitment and activation of the phosphatases PTPN6 and PTPN11. It also reduces the increase of intracellular calcium evoked by B-cell receptor ligation.
Synonyms :
CD305; HLAIR1; LAIR1; LAIR-1
References & Citations :
(1)Clinical significance of LAIR1 (CD305) as assessed by flow cytometry in a prospective series of patients with chronic lymphocytic leukemia[J]. Haematologica, 2014, 99(5):881-887.
Related category websites: https://www.medchemexpress.com/recombinant-proteins.html
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