Name : Human L1CAM Protein
Product Source :
Recombinant Human L1CAM Protein is expressed from HEK293 with His tag at the C-Terminus. It contains Ile20-Glu1120.[Accession | P32004-1]
Molecular Weight :
The protein has a predicted MW of 124.6 kDa. Due to glycosylation, the protein migrates to 150-180 kDa based on Tris-Bis PAGE result.
Endotoxin Level :
Less than 1EU per μg by the LAL method.
Purity :
> 95% as determined by Tris-Bis PAGE> 95% as determined by HPLC
Formulation :
Lyophilized from 0.22μm filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.
Reconstitution :
Centrifuge the tube before opening. Reconstituting to a concentration more than 100 μg/ml is recommended. Dissolve the lyophilized protein in distilled water.
Storage and Stability :
-20 to -80°C for 12 months as supplied from date of receipt.-80°C for 3-6 months after reconstitution.2-8°C for 2-7 days after reconstitution.Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.
Product Concentration :
Tris-Bis PAGE Human L1CAM on Tris-Bis PAGE under reduced condition. The purity is greater than 95%. SEC-HPLC The purity of Human L1CAM is greater than 95% as determined by SEC-HPLC. ELISA Data Immobilized Human L1CAM, His Tag at 0.2μg/ml (100μl/well) on the plate. Dose response curve for Anti-L1CAM Antibody, hFc Tag with the EC50 of 8.7ng/ml determined by ELISA.
Background :
L1 cell adhesion molecule (L1CAM) is one of the first neural adhesion molecules described with important functions in the development of the nervous system. Subsequent work discovered that L1CAM is expressed in many human cancers and is often associated with bad prognosis. This is most likely due to the motility and invasion promoting function of L1CAM. L1CAM is a valuable diagnostic/prognostic marker and an attractive target for the therapy of several human cancers.
Synonyms :
N-CAM-L1; NCAM-L1; CD171; L1CAM; CAML1; MIC5; CALL; CHL1; CHL-1; FLJ44930; L1CAM-2; MGC132578; HSAS; HSAS1; MASA; S10; SPG1
References & Citations :
(1) Altevogt P, Doberstein K, Fogel M. L1CAM in human cancer. Int J Cancer. 2016 Apr 1;138(7):1565-76. doi: 10.1002/ijc.29658. Epub 2015 Aug 25. PMID: 26111503.
Related category websites: https://www.medchemexpress.com/recombinant-proteins.html
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