Name : Mouse LCAT Protein
Product Source :
Recombinant Mouse LCAT Protein is expressed from HEK293 with His tag at the C-Terminus. It contains Phe25-Glu438.[Accession | P16301]
Molecular Weight :
The protein has a predicted MW of 48.3 kDa. Due to glycosylation, the protein migrates to 67-75 kDa based on Tris-Bis PAGE result.
Endotoxin Level :
Less than 1EU per μg by the LAL method.
Purity :
> 95% as determined by Tris-Bis PAGE
Formulation :
Lyophilized from 0.22μm filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.
Reconstitution :
Centrifuge the tube before opening. Reconstituting to a concentration more than 100 μg/ml is recommended. Dissolve the lyophilized protein in distilled water.
Storage and Stability :
-20 to -80°C for 12 months as supplied from date of receipt.-80°C for 3-6 months after reconstitution.2-8°C for 2-7 days after reconstitution.Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.
Product Concentration :
Tris-Bis PAGE Mouse LCAT on Tris-Bis PAGE under reduced condition. The purity is greater than 95%.
Background :
Lecithin:cholesterol acyltransferase (LCAT) is the only enzyme capable of esterifying cholesterol in plasma, thus determining the maturation of high-density lipoproteins. Because it maintains an unesterified cholesterol gradient between peripheral cells and extracellular acceptors, for a long time, LCAT has been considered as a key enzyme in reverse cholesterol transport.
Synonyms :
PAF acetylhydrolase; LCAT
References & Citations :
(1) Ossoli A, Simonelli S, Vitali C, Franceschini G, Calabresi L. Role of LCAT in Atherosclerosis. J Atheroscler Thromb. 2016;23(2):119-27. doi: 10.5551/jat.32854. Epub 2015 Nov 26. PMID: 26607351.
Related category websites: https://www.medchemexpress.com/recombinant-proteins.html
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