Name : Human B7-1/CD80 Protein
Product Source :
Recombinant Human B7-1/CD80 Protein is expressed from HEK293 with His tag and Avi tag at the C-Terminus. It contains Val35-Asn242.[Accession | P33681-1]
Molecular Weight :
The protein has a predicted MW of 26.8 kDa. Due to glycosylation, the protein migrates to 50-70 kDa based on Tris-Bis PAGE result.
Endotoxin Level :
Less than 1EU per μg by the LAL method.
Purity :
> 95% as determined by Tris-Bis PAGE> 95% as determined by HPLC
Formulation :
Lyophilized from 0.22μm filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.
Reconstitution :
Centrifuge the tube before opening. Reconstituting to a concentration more than 100 μg/ml is recommended. Dissolve the lyophilized protein in distilled water.
Storage and Stability :
-20 to -80°C for 12 months as supplied from date of receipt. -80°C for 3-6 months after reconstitution. 2-8°C for 2-7 days after reconstitution. Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.
Product Concentration :
Tris-Bis PAGE Human B7-1 on Tris-Bis PAGE under reduced condition. The purity is greater than 95%. SEC-HPLC The purity of Human B7-1 is greater than 95% as determined by SEC-HPLC. ELISA Data Immobilized Human CTLA-4, hFc Tag at 1μg/ml (100μl/well) on the plate. Dose response curve for Human B7-1, His Tag with the EC50 of 1.48μg/ml determined by ELISA (QC Test). SPR Data Human B7-1, His Tag captured on CM5 Chip via Anti-His Antibody can bind Human CTLA4, hFc Tag with an affinity constant of 1.1nM as determined in SPR assay (Biacore T200).
Background :
Cluster of differentiation 80 (also CD80 and B7-1) is a protein found on dendritic cells, activated B cells and monocytes that provides a costimulatory signal necessary for T cell activation and survival. It is the ligand for two different proteins on the T cell surface: CD28 and CTLA-4.
Synonyms :
CD80 molecule; B7; B71; B7-1; BB1; CD28LG1; CD80; B7.1; CD28LG1; LAB7
References & Citations :
(1) Merwe V D , P. A. CD80 (B7-1) Binds Both CD28 and CTLA-4 with a Low Affinity and Very Fast Kinetics[J]. Journal of Experimental Medicine, 1997, 185(3):393-404.
Related category websites: https://www.medchemexpress.com/recombinant-proteins.html
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