Name : Human LDLR Protein
Product Source :
Recombinant Human LDLR Protein is expressed from HEK293 with His tag and Avi tag at the C-Terminus. It contains Ala22-Arg788.[Accession | P01130-1]
Molecular Weight :
The protein has a predicted MW of 87.6 kDa, Due to glycosylation, the protein migrates to 110-130 kDa based on Tris-Bis PAGE result.
Endotoxin Level :
Less than 1EU per μg by the LAL method.
Purity :
> 95% as determined by Tris-Bis PAGE> 95% as determined by HPLC
Formulation :
Lyophilized from 0.22μm filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.
Reconstitution :
Centrifuge the tube before opening. Reconstituting to a concentration more than 100 μg/ml is recommended. Dissolve the lyophilized protein in distilled water.
Storage and Stability :
-20 to -80°C for 12 months as supplied from date of receipt.-80°C for 3-6 months after reconstitution.2-8°C for 2-7 days after reconstitution.Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.
Product Concentration :
Tris-Bis PAGE Human LDLR on Tris-Bis PAGE under reduced condition. The purity is greater than 95%. SEC-HPLC The purity of Human LDLR is greater than 95% as determined by SEC-HPLC. SPR Data Human LDLR, His Tag immobilized on CM5 Chip can bind Human PCSK9, His Tag with an affinity constant of 0.35 nM as determined in SPR assay (Biacore T200).
Background :
The low density lipoprotein receptor (LDLR) is the founding member of the LDL R family of widely expressed cell surface scavenger receptors. It is a cell-surface receptor that recognizes the apoprotein B100 which is embedded in the phospholipid outer layer of LDL particles.
Synonyms :
LDLR; FHC; LDL R; LDL receptor; LDLCQ2; FH
References & Citations :
(1)Al-Allaf F A , Coutelle C , Waddington S N , et al. LDLR-Gene therapy for familial hypercholesterolaemia: Problems, progress, and perspectives[J]. International Archives of Medicine, 2010, 3(1):36.
Related category websites: https://www.medchemexpress.com/recombinant-proteins.html
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