Name : Mouse Leptin Protein
Product Source :
Recombinant Mouse Leptin Protein is expressed from HEK293 with hFc tag at the C-Terminus. It contains Val22-Cys167.[Accession | P41160]
Molecular Weight :
The protein has a predicted MW of 42.76 kDa. Due to glycosylation, the protein migrates to 45-50 kDa based on Tris-Bis PAGE result.
Endotoxin Level :
Less than 1EU per μg by the LAL method.
Purity :
> 95% as determined by Tris-Bis PAGE> 95% as determined by HPLC
Formulation :
Lyophilized from 0.22μm filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.
Reconstitution :
Centrifuge the tube before opening. Reconstituting to a concentration more than 100 μg/ml is recommended. Dissolve the lyophilized protein in distilled water.
Storage and Stability :
-20 to -80°C for 12 months as supplied from date of receipt.-80°C for 3-6 months after reconstitution.2-8°C for 2-7 days after reconstitution.Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.
Product Concentration :
Tris-Bis PAGE Mouse Leptin on Tris-Bis PAGE under reduced condition. The purity is greater than 95%. SEC-HPLC The purity of Mouse Leptin is greater than 95% as determined by SEC-HPLC.
Background :
Leptin has key roles in the regulation of energy balance, body weight, metabolism, and endocrine function. Leptin levels are undetectable or very low in patients with lipodystrophy, hypothalamic amenorrhea, and congenital Leptin deficiency (CLD) due to mutations in the Leptin gene.
Synonyms :
Leptin; Obese protein; Obesity factor; LEP; OB; OBS; LEPD
References & Citations :
(1)Paz-Filho G, Mastronardi CA, Licinio J. Leptin treatment: facts and expectations. Metabolism. 2015 Jan;64(1):146-56. doi: 10.1016/j.metabol.2014.07.014. Epub 2014 Aug 3. PMID: 25156686.
Related category websites: https://www.medchemexpress.com/recombinant-proteins.html
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