Name : Human LGR-5 Protein
Product Source :
Recombinant Human LGR-5 Protein is expressed from HEK293 with hFc tag at the C-Terminus. It contains Gly22-Pro543.[Accession | O75473-1]
Molecular Weight :
The protein has a predicted MW of 85 kDa. Due to glycosylation, the protein migrates to 110-120 kDa based on Tris-Bis PAGE result.
Endotoxin Level :
Less than 1EU per μg by the LAL method.
Purity :
> 95% as determined by Tris-Bis PAGE
Formulation :
Lyophilized from 0.22μm filtered solution in PBS, 5mM DTT (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.
Reconstitution :
Centrifuge the tube before opening. Reconstituting to a concentration more than 100 μg/ml is recommended. Dissolve the lyophilized protein in distilled water.
Storage and Stability :
-20 to -80°C for 12 months as supplied from date of receipt.-80°C for 3-6 months after reconstitution.2-8°C for 2-7 days after reconstitution.Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.
Product Concentration :
Tris-Bis PAGE Human LGR-5 on Tris-Bis PAGE under reduced condition. The purity is greater than 95%. SPR Data Human LGR-5, hFc Tag captured on CM5 Chip via Protein A can bind Human R-Spondin 3, His Tag with an affinity constant of 0.59 nM as determined in SPR assay (Biacore T200).
Background :
G protein-coupled receptor 5 (LGR5), known as a stem cell marker for colon cancer and gastric cancer, can serve as a novel GSC marker involved in EMT and a therapeutic target in glioma.LGR5 is a new functional GSC marker and prognostic indicator that can promote EMT by activating the Wnt/β-catenin pathway and would thus be a novel therapeutic target for glioma.
Synonyms :
LGR5; GPR49; GPR67; HG38; G-protein coupled receptor 49; G-protein coupled receptor 67; G-protein coupled receptor HG38
References & Citations :
Zhang J, Cai H, Sun L, Zhan P, Chen M, Zhang F, Ran Y, Wan J. LGR5, a novel functional glioma stem cell marker, promotes EMT by activating the Wnt/β-catenin pathway and predicts poor survival of glioma patients. J Exp Clin Cancer Res. 2018 Sep 12;37(1):225. doi: 10.1186/s13046-018-0864-6. PMID: 30208924; PMCID: PMC6136228.
Related category websites: https://www.medchemexpress.com/recombinant-proteins.html
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