Name : Human LIF Protein

Product Source :
Recombinant Human LIF Protein is expressed from E.coli without tag. It contains Ser23-Phe202.[Accession | P15018-1]

Molecular Weight :
The protein has a predicted MW of 19.86 kDa same as Tris-Bis PAGE result.

Endotoxin Level :
Less than 1EU per μg by the LAL method.

Purity :
> 95% as determined by Tris-Bis PAGE> 95% as determined by HPLC

Formulation :
Lyophilized from 0.22 μm filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.

Reconstitution :
Centrifuge the tube before opening. Reconstituting to a concentration more than 100 μg/ml is recommended. Dissolve the lyophilized protein in distilled water.

Storage and Stability :
-20 to -80°C for 12 months as supplied from date of receipt. -80°C for 3-6 months after reconstitution. 2-8°C for 2-7 days after reconstitution. Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.

Product Concentration :
Tris-Bis PAGE Human LIF on Tris-Bis PAGE under reduced condition. The purity is greater than 95%. SEC-HPLC The purity of Human LIF is greater than 95% as determined by SEC-HPLC. ELISA Data Immobilized Human LIF, No Tag at 2μg/ml (100μl/well) on the plate. Dose response curve for Human LIF R, His Tag with the EC50 of 0.30μg/ml determined by ELISA.

Background :
Leukemia inhibitory factor (LIF) has played a vital role in a series of reproductive events, including follicle growth, embryo growth and differentiation. However, it is unclear whether the level of LIF in embryo culture medium can be used as a marker for clinical pregnancy.

Synonyms :
LIF; HILDA; MLPLI; CDF; DIA

References & Citations :
(1) Nicola NA, Babon JJ. Leukemia inhibitory factor (LIF). Cytokine Growth Factor Rev. 2015 Oct;26(5):533-44. doi: 10.1016/j.cytogfr.2015.07.001. Epub 2015 Jul 4. PMID: 26187859; PMCID: PMC4581962.

MedChemExpress (MCE) recombinant proteins include: cytokines, enzymes, growth factors, hormones, receptors, transcription factors, antibody fragments, etc. They are often essential for supporting cell growth, stimulating cell signaling pathways, triggering or inhibiting cell differentiation; and are useful tools for elucidating protein structure and function, understanding disease onset and progression, and validating pharmaceutical targets. At MedChemExpress (MCE), we strive to provide products with only the highest quality. Protein identity, purity and biological activity are assured by our robust quality control and assurance procedures.
Related category websites: https://www.medchemexpress.com/recombinant-proteins.html
Popular product recommendations:
Annexin A6/ANXA6 Protein
CD3D Protein
Popular categories:
BDCA-4/CD304
Pellino-1