Name : Human LIF Protein
Product Source :
Recombinant Human LIF Protein is expressed from HEK293 without tag. It contains Ser23-Phe202.[Accession | P15018-1]
Molecular Weight :
The protein has a predicted MW of 19.7 kDa. Due to glycosylation, the protein migrates to 40-55 kDa based on Tris-Bis PAGE result.
Endotoxin Level :
Less than 1EU per μg by the LAL method.
Purity :
> 95% as determined by Tris-Bis PAGE> 95% as determined by HPLC
Formulation :
Lyophilized from 0.22μm filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.
Reconstitution :
Centrifuge the tube before opening. Reconstituting to a concentration more than 100 μg/ml is recommended. Dissolve the lyophilized protein in distilled water.
Storage and Stability :
-20 to -80°C for 12 months as supplied from date of receipt. -80°C for 3-6 months after reconstitution. 2-8°C for 2-7 days after reconstitution. Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.
Product Concentration :
Tris-Bis PAGE Human LIF on Tris-Bis PAGE under reduced condition. The purity is greater than 95%. SEC-HPLC The purity of Human LIF is greater than 95% as determined by SEC-HPLC. ELISA Data Immobilized Human LIF at 2μg/ml (100μl/Well) on the plate. Dose response curve for Human LIF R, hFc Tag with the EC50 of 55.9ng/ml determined by ELISA. Cell Based Assay Determined by its dose-dependent ability to induce reporter gene in 293T-STAT3-Luc2 reporter cells. The ED50 for this effect is 1-5 ng/mL.
Background :
Leukemia inhibitory factor (LIF) has played a vital role in a series of reproductive events, including follicle growth, embryo growth and differentiation. However, it is unclear whether the level of LIF in embryo culture medium can be used as a marker for clinical pregnancy.
Synonyms :
LIF; HILDA; MLPLI; CDF; DIA
References & Citations :
(1) Nicola NA, Babon JJ. Leukemia inhibitory factor (LIF). Cytokine Growth Factor Rev. 2015 Oct;26(5):533-44. doi: 10.1016/j.cytogfr.2015.07.001. Epub 2015 Jul 4. PMID: 26187859; PMCID: PMC4581962.
Related category websites: https://www.medchemexpress.com/recombinant-proteins.html
Popular product recommendations:
CD7 Protein
KLF6 Protein
Popular categories:
Junctional Adhesion Molecule-Like Protein (JAML)
UCH-L3