Name : Human LIGHT/TNFSF14 Protein
Product Source :
Recombinant Human LIGHT/TNFSF14 Protein is expressed from HEK293 with His tag at the N-Terminus. It contains Asp74-Val240.[Accession | O43557-1]
Molecular Weight :
The protein has a predicted MW of 19 kDa. Due to glycosylation, the protein migrates to 25-30 kDa based on Tris-Bis PAGE result.
Endotoxin Level :
Less than 1EU per μg by the LAL method.
Purity :
> 95% as determined by Tris-Bis PAGE> 95% as determined by HPLC
Formulation :
Lyophilized from 0.22μm filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.
Reconstitution :
Centrifuge the tube before opening. Reconstituting to a concentration more than 100 μg/ml is recommended. Dissolve the lyophilized protein in distilled water.
Storage and Stability :
-20 to -80°C for 12 months as supplied from date of receipt. -80°C for 3-6 months after reconstitution. 2-8°C for 2-7 days after reconstitution. Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.
Product Concentration :
Tris-Bis PAGE Human LIGHT on Tris-Bis PAGE under reduced condition. The purity is greater than 95%. SEC-HPLC The purity of Human LIGHT is greater than 95% as determined by SEC-HPLC. ELISA Data Immobilized Human LIGHT, His Tag at 1μg/ml (100μl/Well) on the plate. Dose response curve for Human HVEM, hFc Tag with the EC50 of 39.9ng/ml determined by ELISA (QC Test). ELISA Data Immobilized Human LIGHT, His Tag at 0.5μg/ml (100μl/Well) on the plate. Dose response curve for Human LTBR, hFc Tag with the EC50 22.9ng/ml determined by ELISA.
Background :
LIGHT, also known as tumor necrosis factor superfamily member 14 (TNFSF14), is a secreted protein of the TNF superfamily. It is recognized by herpesvirus entry mediator (HVEM), as well as decoy receptor 3.This protein has been shown to stimulate the proliferation of T cells, and trigger apoptosis of various tumor cells.
Synonyms :
CD258; TNFSF14; HVEM-L; LIGHT; LTg; TR2
References & Citations :
(1)Granger S W, Rickert S. LIGHT-HVEM signaling and the regulation of T cell-mediated immunity[J]. Cytokine Growth Factor Rev, 2003, 14(3):289-296.
Related category websites: https://www.medchemexpress.com/recombinant-proteins.html
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