Name : Human LRP-5 Protein
Product Source :
Recombinant Human LRP-5 Protein is expressed from HEK293 with mFc (IgG1) tag at the C-Terminus. It contains Glu644-Gln1263.[Accession | O75197-1]
Molecular Weight :
The protein has a predicted MW of 96 kDa. Due to glycosylation, the protein migrates to 100-115 kDa based on Tris-Bis PAGE result.
Endotoxin Level :
Less than 1EU per μg by the LAL method.
Purity :
> 95% as determined by Tris-Bis PAGE
Formulation :
Lyophilized from 0.22μm filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.
Reconstitution :
Centrifuge the tube before opening. Reconstituting to a concentration more than 100 μg/ml is recommended. Dissolve the lyophilized protein in distilled water.
Storage and Stability :
-20 to -80°C for 12 months as supplied from date of receipt.-80°C for 3-6 months after reconstitution.2-8°C for 2-7 days after reconstitution.Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.
Product Concentration :
Tris-Bis PAGE Human LRP-5 on Tris-Bis PAGE under reduced condition. The purity is greater than 95%. ELISA Data Immobilized Human Wnt Surrogate-Fc Fusion, hFc Tag at 5μg/ml (100μl/well) on the plate. Dose response curve for Human LRP-5, mFc Tag with the EC50 of 0.73μg/ml determined by ELISA (QC Test). ELISA Data Immobilized Human DKK1, His Tag at 5μg/ml (100μl/well) on the plate. Dose response curve for Human LRP-5, mFc Tag with the EC50 of 0.11μg/ml determined by ELISA.
Background :
A role for low-density lipoprotein-related receptor 5 (LRP5) in human bone was first established by the identification of genetic alterations that led to dramatic changes in bone mass. Shortly thereafter, mutations that altered the function of the sclerostin (SOST) gene were also associated with altered human bone mass. Subsequent studies of LRP5 and sclerostin have provided important insights into the mechanisms by which these proteins regulate skeletal homeostasis.
Synonyms :
LRP-5; LRP-7; LR3; BMND1; BMND1OPTA1; EVR1; EVR4; HBM; LR3VBCH2; LRP5; LRP7; OPPG; OPS; OPTA1; VBCH2
References & Citations :
(1)Williams BO. LRP5: From bedside to bench to bone. Bone. 2017 Sep;102:26-30. doi: 10.1016/j.bone.2017.03.044. Epub 2017 Mar 21. PMID: 28341377.
Related category websites: https://www.medchemexpress.com/recombinant-proteins.html
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