Name : Human LRRC15/LIB Protein
Product Source :
Recombinant Human LRRC15/LIB Protein is expressed from HEK293 with hFc tag at the C-terminus. It contains Tyr22-Gly538.[Accession | Q8TF66-1]
Molecular Weight :
The protein has a predicted MW of 84.54 kDa. Due to glycosylation, the protein migrates to 90-120 kDa based on Tris-Bis PAGE result.
Endotoxin Level :
Less than 1EU per μg by the LAL method.
Purity :
> 95% as determined by Tris-Bis PAGE> 95% as determined by HPLC
Formulation :
Lyophilized from 0.22 μm filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.
Reconstitution :
Centrifuge the tube before opening. Reconstituting to a concentration more than 100 μg/ml is recommended. Dissolve the lyophilized protein in distilled water.
Storage and Stability :
-20 to -80°C for 12 months as supplied from date of receipt. -80°C for 3 months after reconstitution. Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.
Product Concentration :
Tris-Bis PAGE Human LRRC15 on Tris-Bis PAGE under reduced condition. The purity is greater than 95%. SEC-HPLC The purity of Human LRRC15 is greater than 95% as determined by SEC-HPLC. ELISA Data Immobilized Human LRRC15, hFc Tag at 2μg/ml (100μl/well) on the plate. Dose response curve for Biotinylated Anti-LRRC15 Antibody, hFc Tag with the EC50 of 11.9ng/ml determined by ELISA.
Background :
The chimeric protein binds in vitro and in vivo to a specific element upstream of LRRC15, leading to dramatic transcriptional activation. LRRC15 encodes a leucine-rich transmembrane protein, present at the leading edge of migrating cells, the expression of which in normal tissues is restricted to the invasive cytotrophoblast layer of the placenta; small interfering (siRNA)-mediated suppression of LRRC15 expression in breast cancer cells leads to abrogation of invasiveness in vitro.
Synonyms :
LIB; LRRC15
References & Citations :
(1)Kobayashi I, Kobayashi-Sun J, Hirakawa Y, et al. Dual role of Jam3b in early hematopoietic and vascular development. Development. 2020;147(1):dev181040. Published 2020 Jan 8. doi:10.1242/dev.181040
Related category websites: https://www.medchemexpress.com/recombinant-proteins.html
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