Name : Human CD229/SLAMF3 Protein

Product Source :
Recombinant Human CD229/SLAMF3 Protein is expressed from HEK293 with mFc (IgG1) tag at the C-terminus. It contains Gly251-Asn452.[Accession | Q9HBG7-1]

Molecular Weight :
The protein has a predicted MW of 48.04 kDa. Due to glycosylation, the protein migrates to 55-70 kDa based on Tris-Bis PAGE result.

Endotoxin Level :
Less than 1EU per μg by the LAL method.

Purity :
> 95% as determined by Tris-Bis PAGE> 95% as determined by HPLC

Formulation :
Supplied as 0.22 μm filtered solution in PBS (pH 7.4).

Reconstitution :

Storage and Stability :
Valid for 12 months from date of receipt when stored at -80°C. Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.

Product Concentration :
Tris-Bis PAGE Human CD229 on Tris-Bis PAGE under reduced condition. The purity is greater than 95%. SEC-HPLC The purity of Human CD229 is greater than 95% as determined by SEC-HPLC.

Background :
CD229 was strongly and homogeneously overexpressed on the PC of patients with monoclonal gammopathy of undetermined significance (MGUS), smoldering myeloma, MM, and PC leukemia. CD229 was particularly overexpressed on those PC showing an abnormal phenotype such as expression of CD56. Most importantly, CD229 was also highly expressed on those cells in the patients’ BM displaying the phenotype of chemotherapy-resistant and myeloma-propagating cells.

Synonyms :
SLAMF3; CD229; LY9; AI893573; Lgp100; T100

References & Citations :
(1) Yousef S, Kovacsovics-Bankowski M, Salama ME, Bhardwaj N, Steinbach M, Langemo A, Kovacsovics T, Marvin J, Binder M, Panse J, Kröger N, Luetkens T, Atanackovic D. CD229 is expressed on the surface of plasma cells carrying an aberrant phenotype and chemotherapy-resistant precursor cells in multiple myeloma. Hum Vaccin Immunother. 2015;11(7):1606-11. doi: 10.1080/21645515.2015.1046658. PMID: 26001047; PMCID: PMC4514151.

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