Name : Human LY75/CD205 Protein
Product Source :
Recombinant Human LY75/CD205 Protein is expressed from HEK293 with His tag at the C-Terminus. It contains Ser28-Asp1666.[Accession | O60449-1]
Molecular Weight :
The protein has a predicted MW of 190.2 kDa. Due to glycosylation, the protein migrates to 230-245 kDa based on Tris-Bis PAGE result.
Endotoxin Level :
Less than 1EU per ug by the LAL method.
Purity :
> 95% as determined by Tris-Bis PAGE> 95% as determined by HPLC
Formulation :
Lyophilized from 0.22μm filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.
Reconstitution :
Centrifuge the tube before opening. Reconstituting to a concentration more than 100 μg/ml is recommended. Dissolve the lyophilized protein in distilled water.
Storage and Stability :
-20 to -80°C for 12 months as supplied from date of receipt.-80°C for 3-6 months after reconstitution.2-8°C for 2-7 days after reconstitution.Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.
Product Concentration :
Tris-Bis PAGE Human LY75 on Tris-Bis PAGE under reduced condition. The purity is greater than 95%. SEC-HPLC The purity of Human LY75 is greater than 95% as determined by SEC-HPLC. ELISA Data Immobilized Human LY75, His Tag at 0.1μg/ml (100μl/well) on the plate. Dose response curve for Anti-LY75 Antibody, hFc Tag with the EC50 of 5.1ng/ml determined by ELISA.
Background :
Lymphocyte antigen 75 (Ly75/CD205) is a surface marker on mitotic germ cells in rainbow trout. In mammals, several cell surface molecular markers have been characterized in order to identify the mitotic germ cells. he expression profile of Ly75 protein was similar to that of the mRNA. Furthermore, identification of various fish homologs of ly75 confirmed that their amino acid sequences are well conserved. Therefore, Ly75 may be appropriate for use as a versatile cell surface marker for mitotic germ cells in fish.
Synonyms :
Ly-75; DEC-205; CD205; CLEC13B; GP200-MR6
References & Citations :
(1) Nagasawa K, Shikina S, Takeuchi Y, Yoshizaki G. Lymphocyte antigen 75 (Ly75/CD205) is a surface marker on mitotic germ cells in rainbow trout. Biol Reprod. 2010 Oct;83(4):597-606. doi: 10.1095/biolreprod.109.082081. Epub 2010 Jun 10. PMID: 20554922.
Related category websites: https://www.medchemexpress.com/recombinant-proteins.html
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