Name : Mouse LY6A Protein

Product Source :
Recombinant Mouse LY6A Protein is expressed from E.coli with His tag at the N-terminus. It contains Leu27-Gly119.[Accession | NP_001258345.1]

Molecular Weight :
The protein has a predicted MW of 10.80 kDa. The protein migrates to 15-20 kDa based on Tris-Bis PAGE result.

Endotoxin Level :
Less than 1EU per μg by the LAL method.

Purity :
> 95% as determined by Tris-Bis PAGE> 95% as determined by HPLC

Formulation :
Lyophilized from 0.22μm filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.

Reconstitution :
Centrifuge the tube before opening. Reconstituting to a concentration more than 100 μg/ml is recommended. Dissolve the lyophilized protein in distilled water.

Storage and Stability :
-20 to -80°C for 12 months as supplied from date of receipt.-80°C for 3-6 months after reconstitution.2-8°C for 2-7 days after reconstitution.Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.

Product Concentration :
Tris-Bis PAGE Mouse LY6A on Tris-Bis PAGE under reduced condition. The purity is greater than 95%. SEC-HPLC The purity of Mouse LY6A is greater than 95% as determined by SEC-HPLC.

Background :
Stem Cell Antigen-1 (Sca-1/Ly6A) was the first identified member of the Lymphocyte antigen-6 (Ly6) gene family. Sca-1 is a cell surface glycoprotein that is often used as a biomarker for stem cells and cell stemness.

Synonyms :
Ly-6A.2; Ly-6E.1; SCA-1; SCA 1; TAP; Ly6

References & Citations :
Upadhyay G. Emerging Role of Lymphocyte Antigen-6 Family of Genes in Cancer and Immune Cells. Front Immunol. 2019 Apr 24;10:819. doi: 10.3389/fimmu.2019.00819. PMID: 31068932; PMCID: PMC6491625.

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