Name : Human LY6E Protein
Product Source :
Recombinant Human LY6E Protein is expressed from HEK293 with mFc tag at the C-terminus. It contains Leu21-Ser101.[Accession | Q16553]
Molecular Weight :
The protein has a predicted MW of 34.15 kDa. Due to glycosylation, the protein migrates to 40-48 kDa based on Tris-Bis PAGE result.
Endotoxin Level :
Less than 1EU per μg by the LAL method.
Purity :
> 95% as determined by Tris-Bis PAGE> 95% as determined by HPLC
Formulation :
Lyophilized from 0.22 μm filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.
Reconstitution :
Centrifuge the tube before opening. Reconstituting to a concentration more than 100 μg/ml is recommended. Dissolve the lyophilized protein in distilled water.
Storage and Stability :
-20 to -80°C for 12 months as supplied from date of receipt.-80°C for 3-6 months after reconstitution.2-8°C for 2-7 days after reconstitution.Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.
Product Concentration :
Tris-Bis PAGE Human LY6E on Tris-Bis PAGE under reduced condition. The purity is greater than 95%. SEC-HPLC The purity of Human LY6E is greater than 95% as determined by SEC-HPLC. ELISA Data Immobilized Human LY6E, mFc Tag at 1μg/ml (100μl/well) on the plate. Dose response curve for Anti-LY6E Antibody, hFc Tag with the EC50 of 10.6ng/ml determined by ELISA.
Background :
Lymphocyte antigen 6 complex, locus E (LY6E), also designated as stem cell antigen 2 (SCA2) and thymic shared antigen-1 (TSA-1), is a member of the lymphostromal cell membrane Ly6 superfamily. All Ly6 family members are low-molecular weight (10-12 kDa) proteins that display limited nucleotide and amino acid sequence homologies with one another, but commonly have a conserved cysteine-rich cell surface domain and are anchored to the cell surface via a glycosilphosphatidylinositol (GPI) moiety.
Synonyms :
Ly-6E; RIG-E; SCA-2; TSA-1; 9804; RIGE; SCA2; TSA1
References & Citations :
Yeom CJ, Zeng L, Goto Y, Morinibu A, Zhu Y, Shinomiya K, Kobayashi M, Itasaka S, Yoshimura M, Hur CG, Kakeya H, Hammond EM, Hiraoka M, Harada H. LY6E: a conductor of malignant tumor growth through modulation of the PTEN/PI3K/Akt/HIF-1 axis. Oncotarget. 2016 Oct 4;7(40):65837-65848. doi: 10.18632/oncotarget.11670. PMID: 27589564; PMCID: PMC5323196.
Related category websites: https://www.medchemexpress.com/recombinant-proteins.html
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