Name : Monkeypox virus M1R Protein

Product Source :
Recombinant Monkeypox virus M1R Protein is expressed from HEK293 with His tag at the C-Terminus. It contains Gly2-Gly183.[Accession | QJQ40223.1]

Molecular Weight :
The protein has a predicted MW of 20.41 kDa. Due to glycosylation, the protein migrates to 23-38 kDa based on Tris-Bis PAGE result.

Endotoxin Level :
Less than 1EU per μg by the LAL method.

Purity :
> 95% as determined by Tris-Bis PAGE> 95% as determined by HPLC

Formulation :
Lyophilized from 0.22μm filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.

Reconstitution :
Centrifuge the tube before opening. Reconstituting to a concentration more than 100 μg/ml is recommended. Dissolve the lyophilized protein in distilled water.

Storage and Stability :
-20 to -80°C for 12 months as supplied from date of receipt.-80°C for 3-6 months after reconstitution.2-8°C for 2-7 days after reconstitution.Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.

Product Concentration :
Tris-Bis PAGE Monkeypox virus M1R on Tris-Bis PAGE under reduced condition. The purity is greater than 95%. SEC-HPLC The purity of Monkeypox virus M1R is greater than 95% as determined by SEC-HPLC.

Background :
Monkeypox virus (MPXV) is double-stranded DNA virus belonging to the genus orthopoxvirus that causes a smallpox-like disease in humans.

Synonyms :
M1R; Monkeypox virus

References & Citations :
(1) Alakunle E, Moens U, Nchinda G, Okeke MI. Monkeypox Virus in Nigeria: Infection Biology, Epidemiology, and Evolution. Viruses. 2020 Nov 5;12(11):1257. doi: 10.3390/v12111257. PMID: 33167496; PMCID: PMC7694534.

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