Name : Cynomolgus MFAP4 Protein
Product Source :
Recombinant Cynomolgus MFAP4 Protein is expressed from HEK293 with His tag at the C-Terminus. It contains Val60-Ala293.[Accession | A0A2K5TQS8]
Molecular Weight :
The protein has a predicted MW of 27.60 kDa. Due to glycosylation, the protein migrates to 35-40 kDa based on Tris-Bis PAGE result.
Endotoxin Level :
Less than 1EU per μg by the LAL method.
Purity :
> 95% as determined by Tris-Bis PAGE> 95% as determined by HPLC
Formulation :
Lyophilized from 0.22μm filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.
Reconstitution :
Centrifuge the tube before opening. Reconstituting to a concentration more than 100 μg/ml is recommended. Dissolve the lyophilized protein in distilled water.
Storage and Stability :
-20 to -80°C for 12 months as supplied from date of receipt.-80°C for 3-6 months after reconstitution.2-8°C for 2-7 days after reconstitution.Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.
Product Concentration :
Tris-Bis PAGE Cynomolgus MFAP4 on Tris-Bis PAGE under reduced condition. The purity is greater than 95%. SEC-HPLC The purity of Cynomolgus MFAP4 is greater than 95% as determined by SEC-HPLC. ELISA Data Immobilized Cynomolgus MFAP4, His Tag at 1μg/ml (100μl/Well) on the plate. Dose response curve for Anti-MFAP4 Antibody, hFc Tag with the EC50 of 7.8ng/ml determined by ELISA.
Background :
Microfibril-associated glycoprotein 4 (MFAP4) is an extracellular matrix protein belonging to the fibrinogen-related protein superfamily. MFAP4 is produced by vascular smooth muscle cells and is highly enriched in the blood vessels of the heart and lung, where it is thought to contribute to the structure and function of elastic fibers. Genetic studies in humans have implicated MFAP4 in the pathogenesis of Smith-Magenis syndrome, in which patients present with multiple congenital abnormalities and mental retardation, as well as in the severe cardiac malformation left-sided congenital heart disease.
Synonyms :
Microfibril-associated glycoprotein 4;Mfap4
References & Citations :
(1) Ong SLM, de Vos IJHM, Meroshini M, Poobalan Y, Dunn NR. Microfibril-associated glycoprotein 4 (Mfap4) regulates haematopoiesis in zebrafish. Sci Rep. 2020 Jul 16;10(1):11801. doi: 10.1038/s41598-020-68792-8. PMID: 32678226; PMCID: PMC7366704.
Related category websites: https://www.medchemexpress.com/recombinant-proteins.html
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