Name : Human MANSC1 Protein
Product Source :
Recombinant Human MANSC1 Protein is expressed from HEK293 with His tag at the C-Terminus. It contains Gln27-Leu385.[Accession | Q9H8J5]
Molecular Weight :
The protein has a predicted MW of 39.9 kDa. Due to glycosylation, the protein migrates to 113-116 kDa based on Tris-Bis PAGE result.
Endotoxin Level :
Less than 1EU per μg by the LAL method.
Purity :
> 95% as determined by Tris-Bis PAGE> 95% as determined by HPLC
Formulation :
Lyophilized from 0.22μm filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.
Reconstitution :
Centrifuge the tube before opening. Reconstituting to a concentration more than 100 μg/ml is recommended. Dissolve the lyophilized protein in distilled water.
Storage and Stability :
-20 to -80°C for 12 months as supplied from date of receipt.-80°C for 3-6 months after reconstitution.2-8°C for 2-7 days after reconstitution.Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.
Product Concentration :
Tris-Bis PAGE Human MANSC1 on Tris-Bis PAGE under reduced condition. The purity is greater than 95%. SEC-HPLC The purity of Human MANSC1 is greater than 95% as determined by SEC-HPLC. ELISA Data Immobilized Human MANSC1, His Tag at 0.2μg/ml (100μl/well) on the plate. Dose response curve for Anti-MANSC1 Antibody, hFc Tag with the EC50 of 7.5ng/ml determined by ELISA.
Background :
MANSC1 contains 1 MANSC domain. MANSC is a seven-cysteine-containing domain present in animal membrane and extracellular proteins. MANSC (motif at N terminus with seven cysteines) is a novel domain with a well-conserved seven-cysteine motif that is present at the N terminus of membrane and extracellular proteins, including low-density lipoprotein receptor-related protein 11 (LRP-11), hepatocyte growth factor activator inhibitor 1 (HAI-1) and some uncharacterized proteins encoded by multicellular animals from Mollusca to Chordata. The MANSC domain in HAI-2 might function through binding with hepatocyte growth factor activator and matriptase[1].
Synonyms :
MANSC1; LOH12CR3; 9130403P13Rik
References & Citations :
(1)Guo J, Chen S, Huang C, Chen L, Studholme DJ, Zhao S, Yu L. MANSC: a seven-cysteine-containing domain present in animal membrane and extracellular proteins. Trends Biochem Sci. 2004 Apr;29(4):172-4.
Related category websites: https://www.medchemexpress.com/recombinant-proteins.html
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