Name : Mouse MFAP5 Protein
Product Source :
Recombinant Mouse MFAP5 Protein is expressed from HEK293 with hFc tag at the C-Terminus. It contains Gln29-Leu164.[Accession | Q9QZJ6]
Molecular Weight :
The protein has a predicted MW of 42.4 kDa. Due to furin cleavage and glycosylation, the protein migrates to 32-38 kDa and 50-65 kDa based on Tris-Bis PAGE result.
Endotoxin Level :
Less than 1EU per μg by the LAL method.
Purity :
> 95% as determined by Tris-Bis PAGE> 95% as determined by HPLC
Formulation :
Lyophilized from 0.22μm filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.
Reconstitution :
Centrifuge the tube before opening. Reconstituting to a concentration more than 100 μg/ml is recommended. Dissolve the lyophilized protein in distilled water.
Storage and Stability :
-20 to -80°C for 12 months as supplied from date of receipt.-80°C for 3-6 months after reconstitution.2-8°C for 2-7 days after reconstitution.Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.
Product Concentration :
Tris-Bis PAGE Mouse MFAP5 on Tris-Bis PAGE under reduced condition. The purity is greater than 95%. SEC-HPLC The purity of Mouse MFAP5 is greater than 95% as determined by SEC-HPLC.
Background :
Human basal-like breast cancer (BLBC) is an aggressive malignancy with poor prognosis. Since most current treatments are ineffective, there is an urgent need to identify therapeutic targets for BLBC. Microfibrillar-associated protein 5 (MFAP5) plays an important role in the integration of elastic microfibers and the regulation of endothelial cell behaviors.MFAP5 was significantly overexpressed in BLBC tissues and associated with poor metastasis-free survival of patients with BLBC.
Synonyms :
MFAP-5; MAGP-2; MAGP2; MP25; AAT9
References & Citations :
(1) Wu Y, Wu P, Zhang Q, Chen W, Liu X, Zheng W. MFAP5 promotes basal-like breast cancer progression by activating the EMT program. Cell Biosci. 2019 Mar 7;9:24. doi: 10.1186/s13578-019-0284-0. PMID: 30899449; PMCID: PMC6407223.
Related category websites: https://www.medchemexpress.com/recombinant-proteins.html
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