Name : Mouse MEPE Protein
Product Source :
Recombinant Mouse MEPE Protein is expressed from HEK293 with His tag at the C-Terminus. It contains Ala25-Asp441.[Accession | Q8K4L6]
Molecular Weight :
The protein has a predicted MW of 45.3 kDa. The protein is cleaved into 2 chains and due to glycosylation, it migrates to 45-60 kDa based on Tris-Bis PAGE result.
Endotoxin Level :
Less than 1EU per μg by the LAL method.
Purity :
> 95% as determined by Tris-Bis PAGE
Formulation :
Lyophilized from 0.22μm filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.
Reconstitution :
Centrifuge the tube before opening. Reconstituting to a concentration more than 100 μg/ml is recommended. Dissolve the lyophilized protein in distilled water.
Storage and Stability :
-20 to -80°C for 12 months as supplied from date of receipt.-80°C for 3-6 months after reconstitution.2-8°C for 2-7 days after reconstitution.Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.
Product Concentration :
Tris-Bis PAGE Mouse MEPE on Tris-Bis PAGE under reduced condition. The purity is greater than 95%.
Background :
Matrix extracellular phosphoglycoprotein (MEPE) is expressed in bone and teeth where it has multiple functions. The C-terminus of MEPE contains a mineral-binding, acidic serine- and aspartate-rich motif (ASARM) that is also present in other noncollagenous proteins of mineralized tissues.MEPE-derived ASARM peptides function in phosphate homeostasis and direct inhibition of bone mineralization in a phosphorylation-dependent manner.
Synonyms :
OF45; MEPE; Osteoregulin
References & Citations :
(1) Christensen B, Schytte GN, Scavenius C, Enghild JJ, McKee MD, Sørensen ES. FAM20C-Mediated Phosphorylation of MEPE and Its Acidic Serine- and Aspartate-Rich Motif. JBMR Plus. 2020 Jun 26;4(8):e10378. doi: 10.1002/jbm4.10378. PMID: 32803110; PMCID: PMC7422707.
Related category websites: https://www.medchemexpress.com/recombinant-proteins.html
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