Name : SARS-CoV-2 Spike RBD (Omicron BA.4/BA.5/BA.5.1.3/BA.5.2) Protein

Product Source :
Recombinant SARS-CoV-2 Spike RBD (Omicron BA.4/BA.5/BA.5.1.3/BA.5.2) Protein is expressed from HEK293 with His tag at the C-Terminus. It contains Arg319-Phe541(G339D, S371F, S373P, S375F, T376A, D405N, R408S, K417N, N440K, L452R, S477N, T478K, E484A, F486V, Q498R, N501Y, Y505H).[Accession | QHD43416.1]

Molecular Weight :
The protein has a predicted MW of 26.05 kDa. Due to glycosylation, the protein migrates to 35-45 kDa based on Tris-Bis PAGE result.

Endotoxin Level :
Less than 1EU per μg by the LAL method.

Purity :
> 95% as determined by Tris-Bis PAGE> 95% as determined by HPLC

Formulation :
Lyophilized from 0.22μm filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.

Reconstitution :
Centrifuge the tube before opening. Reconstituting to a concentration more than 100 μg/ml is recommended. Dissolve the lyophilized protein in distilled water.

Storage and Stability :
-20 to -80°C for 12 months as supplied from date of receipt.-80°C for 3-6 months after reconstitution.4-8°C for 2-7 days after reconstitution.Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.

Product Concentration :
Tris-Bis PAGE SARS-CoV-2 Spike RBD (Omicron BA.4/BA.5/BA.5.1.3/BA.5.2) on Tris-Bis PAGE under reduced condition. The purity is greater than 95%. SEC-HPLC The purity of SARS-CoV-2 Spike RBD (Omicron BA.4/BA.5/BA.5.1.3/BA.5.2) is greater than 95% as determined by SEC-HPLC. ELISA Data Immobilized SARS-CoV-2 Spike RBD (Omicron BA.4/BA.5/BA.5.1.3/BA.5.2) , His Tag at 1μg/ml (100μl/Well) on the plate. Dose response curve for Human ACE2, hFc Tag with the EC50 of 17.3ng/ml determined by ELISA.

Background :
The spike protein (S) of coronavirus (CoV) attaches the virus to its cellular receptor, angiotensin-converting enzyme 2 (ACE2). A defined receptor-binding domain (RBD) on S mediates this interaction.The S protein plays key parts in the induction of neutralizing-antibody and T-cell responses, as well as protective immunity.

Synonyms :
S protein RBD; S glycoprotein RBD; Spike protein RBD

References & Citations :
(1)Larry J Anderson, Ralph S Baric. Emerging Human Coronaviruses – Disease Potential and Preparedness[J]. N Engl J Med, 2012, 367(19):1850-1852.

MedChemExpress (MCE) recombinant proteins include: cytokines, enzymes, growth factors, hormones, receptors, transcription factors, antibody fragments, etc. They are often essential for supporting cell growth, stimulating cell signaling pathways, triggering or inhibiting cell differentiation; and are useful tools for elucidating protein structure and function, understanding disease onset and progression, and validating pharmaceutical targets. At MedChemExpress (MCE), we strive to provide products with only the highest quality. Protein identity, purity and biological activity are assured by our robust quality control and assurance procedures.
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