Name : Human HLA-E*01:03&B2M&Peptide (VMAPKTLVL) Monomer Negative Control Protein

Product Source :
Recombinant Human HLA-E*01:03&B2M&Peptide (VMAPKTLVL) Monomer Negative Control Protein is expressed from HEK293 with His tag and Avi at the C-Terminus. It contains Gly25-Ile305(HLA-E*01:03), Ile21-Met119(B2M) and VMAPKTLVL peptide.[Accession | P13747(HLA-E*01:03)&P61769(B2M)&VMAPKTLVL]

Molecular Weight :
The protein has a predicted MW of 50.2 kDa. Due to glycosylation, the protein migrates to 52-62 kDa based on Tris-Bis PAGE result.

Endotoxin Level :
Less than 1EU per μg by the LAL method.

Purity :
> 95% as determined by Tris-Bis PAGE> 95% as determined by HPLC

Formulation :
Lyophilized from 0.22 μm filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.

Reconstitution :
Centrifuge the tube before opening. Reconstituting to a concentration more than 100 μg/ml is recommended. Dissolve the lyophilized protein in distilled water.

Storage and Stability :
-20 to -80°C for 12 months as supplied from date of receipt. -80°C for 3-6 months after reconstitution. 2-8°C for 2-7 days after reconstitution. Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.

Product Concentration :
Tris-Bis PAGE Human HLA-E*01:03&B2M&Peptide (VMAPKTLVL) Monomer Negative Control on Tris-Bis PAGE under reduced condition. The purity is greater than 95%. SEC-HPLC The purity of Human HLA-E*01:03&B2M&Peptide (VMAPKTLVL) Monomer Negative Control is greater than 95% as determined by SEC-HPLC.

Background :
HLA-E is a nonclassical member of the major histocompatibility complex class I gene locus. HLA-E protein shares a high level of homology with MHC Ia classical proteins: it has similar tertiary structure, associates with β2-microglobulin, and is able to present peptides to cytotoxic lymphocytes. The main function of HLA-E under normal conditions is to present peptides derived from the leader sequences of classical HLA class I proteins, thus serving for monitoring of expression of these molecules performed by cytotoxic lymphocytes.

Synonyms :
HLAE; sHLA-E; HLAE; MHC class I antigen E; MHC; QA1

References & Citations :
(1) Kanevskiy L, Erokhina S, Kobyzeva P, Streltsova M, Sapozhnikov A, Kovalenko E. Dimorphism of HLA-E and its Disease Association. Int J Mol Sci. 2019 Nov 4;20(21):5496. doi: 10.3390/ijms20215496. PMID: 31690066; PMCID: PMC6862560.

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