Name : Human HLA-A*02:01&B2M&MAGE-A1 (KVLEYVIKV) Monomer Protein
Product Source :
Recombinant Human HLA-A*02:01&B2M&MAGE-A1 (KVLEYVIKV) Monomer Protein is expressed from HEK293 with His tag and Avi tag at the C-Terminus. It contains Gly25-Thr305(HLA-A*02:01), Ile21-Met119(B2M) and KVLEYVIKV peptide.[Accession | A0A140T913(HLA-A*02:01)&P61769(B2M)&KVLEYVIKV]
Molecular Weight :
The protein has a predicted MW of 50.50 kDa. Due to glycosylation, the protein migrates to 53-65 kDa based on Tris-Bis PAGE result.
Endotoxin Level :
Less than 1EU per μg by the LAL method.
Purity :
> 95% as determined by Tris-Bis PAGE> 95% as determined by HPLC
Formulation :
Lyophilized from 0.22 μm filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.
Reconstitution :
Centrifuge the tube before opening. Reconstituting to a concentration more than 100 μg/ml is recommended. Dissolve the lyophilized protein in distilled water.
Storage and Stability :
-20 to -80°C for 12 months as supplied from date of receipt. -80°C for 3-6 months after reconstitution. 2-8°C for 2-7 days after reconstitution. Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.
Product Concentration :
Tris-Bis PAGE Human HLA-A*02:01&B2M&MAGE-A1 (KVLEYVIKV) Monomer on Tris-Bis PAGE under reduced condition. The purity is greater than 95%. SEC-HPLC The purity of Human HLA-A*02:01&B2M&MAGE-A1 (KVLEYVIKV) Monomer is greater than 95% as determined by SEC-HPLC. SPR Data Human HLA-A*02:01&B2M&MAGE-A1 (KVLEYVIKV) Monomer, His Tag captured on CM5 Chip via Anti-His Antibody can bind HLA-A*02:01&B2M&MAGE-A1 TCR with an affinity constant of 116.2 nM as determined in SPR assay (Biacore T200).
Background :
MAGE-A1 belongs to the chromosome X-clustered genes of cancer-testis antigen family and is normally expressed in the human germ line but is also overexpressed in various tumors.
Synonyms :
MAGE-A1; MAGE A1; CT1.1; MAGE-1 antigen; MAGE-1; MZ2-E; MAGE1; MAGE1A
References & Citations :
Wang D, Wang J, Ding N, Li Y, Yang Y, Fang X, Zhao H. MAGE-A1 promotes melanoma proliferation and migration through C-JUN activation. Biochem Biophys Res Commun. 2016 May 13;473(4):959-965. doi: 10.1016/j.bbrc.2016.03.161. Epub 2016 Apr 1. PMID: 27045082.
Related category websites: https://www.medchemexpress.com/recombinant-proteins.html
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