Name : Biotinylated Human Peptide Ready HLA-A*03:01&B2M Monomer Protein
Product Source :
Recombinant Biotinylated Human Peptide Ready HLA-A*03:01&B2M Monomer Protein is expressed from HEK293 with His tag and Avi tag at the C-Terminus. It contains Gly25-Thr305(HLA-A*03:01) and Ile21-Met119(B2M).[Accession | NP_002107.3(HLA-A*03:01)&P61769(B2M)]
Molecular Weight :
The protein has a predicted MW of 48.40 kDa. Due to glycosylation, the protein migrates to 50-60 kDa based on Tris-Bis PAGE result.
Endotoxin Level :
Less than 1EU per μg by the LAL method.
Purity :
> 95% as determined by Tris-Bis PAGE> 95% as determined by HPLC
Formulation :
Lyophilized from 0.22 μm filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.
Reconstitution :
Centrifuge the tube before opening. Reconstituting to a concentration more than 100 μg/ml is recommended. Dissolve the lyophilized protein in distilled water.
Storage and Stability :
-20 to -80°C for 12 months as supplied from date of receipt.-80°C for 3-6 months after reconstitution.2-8°C for 2-7 days after reconstitution.Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.
Product Concentration :
Tris-Bis PAGE Biotinylated Human Peptide Ready HLA-A*03:01&B2M Monomer on Tris-Bis PAGE under reduced condition. The purity is greater than 95%. SEC-HPLC The purity of Biotinylated Human Peptide Ready HLA-A*03:01&B2M Monomer is greater than 95% as determined by SEC-HPLC. ELISA Data Immobilized Biotinylated Human Peptide Ready HLA-A*03:01&B2M Monomer, His Tag at 0.5μg/ml (100μl/well) on the streptavidin precoated plate(5μg/ml). Dose response curve for Anti-HLA class I (W6/32) Antibody, hFc Tag with the EC50 of 7.1ng/ml determined by ELISA.
Background :
Peptide Ready HLA-A*03:01&B2M Monomer is absent from peptide, namely peptide-receptive MHC. It can be loaded with antigenic peptides matching HLA-A*03:01. Peptide ready MHC molecules comprising human HLA alleles and B2M, which can be readily tetramerized and loaded with peptides of choice in a high-throughput manner.
Synonyms :
MHC; HLA-A*03:01; Peptide Ready
References & Citations :
[1] Moritz A, Anjanappa R, Wagner C, Bunk S, Hofmann M, Pszolla G, Saikia A, Garcia-Alai M, Meijers R, Rammensee HG, Springer S, Maurer D. High-throughput peptide-MHC complex generation and kinetic screenings of TCRs with peptide-receptive HLA-A*02:01 molecules. Sci Immunol. 2019 Jul 19;4(37):eaav0860.
Related category websites: https://www.medchemexpress.com/recombinant-proteins.html
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