Name : Human HLA-A*02:01&B2M&CMV pp65 (NLVPMVATV) Monomer Protein
Product Source :
Recombinant Human HLA-A*02:01&B2M&CMV pp65 (NLVPMVATV) Monomer Protein is expressed from HEK293 with His tag and Avi tag at the C-Terminus. It contains Gly25-Thr305(HLA-A*02:01), Ile21-Met119(B2M) and NLVPMVATV peptide.[Accession | A0A140T913(HLA-A*02:01)&P61769(B2M)&NLVPMVATV]
Molecular Weight :
The protein has a predicted MW of 50.35 kDa. Due to glycosylation, the protein migrates to 53-63 kDa based on Tris-Bis PAGE result.
Endotoxin Level :
Less than 1EU per μg by the LAL method.
Purity :
> 95% as determined by Tris-Bis PAGE> 95% as determined by HPLC
Formulation :
Lyophilized from 0.22 μm filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.
Reconstitution :
Centrifuge the tube before opening. Reconstituting to a concentration more than 100 μg/ml is recommended. Dissolve the lyophilized protein in distilled water.
Storage and Stability :
-20 to -80°C for 12 months as supplied from date of receipt. -80°C for 3-6 months after reconstitution. 2-8°C for 2-7 days after reconstitution. Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.
Product Concentration :
Tris-Bis PAGE Human HLA-A*02:01&B2M&CMV pp65 (NLVPMVATV) Monomer on Tris-Bis PAGE under reduced condition. The purity is greater than 95%. SEC-HPLC The purity of Human HLA-A*02:01&B2M&CMV pp65 (NLVPMVATV) Monomer is greater than 95% as determined by SEC-HPLC.
Background :
Human cytomegalovirus (CMV) is one of the major causes of severe complications in immunocompromised patients, such as recipients of hematopoietic cells, solid organ transplantation, and HIV-infected individuals. CMV is also a major pathogen in congenital infection. It can cause life-threatening diseases and severe neurological sequelae in new_x005f_x0002_born infants. CMV phosphoprotein pp65 is thought to be a major antigen for CMV-specific cellular immunity.
Synonyms :
MHC; HLA-A*02:01; CMV pp65
References & Citations :
Tanaka N, Kimura H, Hoshino Y, Nishikawa K, Kojima S, Nishiyama Y, Morishima T. Expression of tegument protein pp65 of human cytomegalovirus (CMV) and its application to the analysis of viral-specific cellular immunity in CMV-infected individuals. Arch Virol. 2002 Dec;147(12):2405-17. doi: 10.1007/s00705-002-0860-0. PMID: 12491106.
Related category websites: https://www.medchemexpress.com/recombinant-proteins.html
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